产品介绍９/div>
产品说明
特异?/p>
Reacts with the human integrin beta1 subunit. Specificity verified by preclearing of beta1in immunoprecipitation, flow cytometry on transfectant cells displaying human beta1 integrin, and reactivity with purified beta1. Recognizes an epitope cluster distinct from MAB2250 and MAB2251(Wilkins et al., 1996).
免疫厞/p>
Jurkat T-leukemic cell line
应用
Research Category
Cell Structure
This Anti-Integrin 1 Antibody, clone N29 (Azide Free) is validated for use in Flow Cytometry and Immunohistochemistry and Immunoprecipitation and Affects Function and Cell Culture and Western Blotting for the detection of Integrin 1.
Research Sub Category
Integrins
Immunohistochemistry (Paraffin) Analysis:A 1:50-2,000 dilution from a representative lot detected Integrin 1 in the membrane of tubule epithelial cells and cells of the glomeruli in human kidney tissue, membrane of alveolar cells, endothelial cells, and bronchiole epithelial cells of human lung tissue.
Immunoprecipitation:A representative lot of this antibody clone was used in immunoprecipitation.
Immunohistochemistry:A representative lot of this antibody clone was used in immunohistochemistry on frozen sections.
Flow Cytometry:A representative lot of this antibody clone was used in flow cytometry.
Functional Activity Assay:A representative lot of this antibody clone was used to stimulate adhesion of cells to extracellular matrix proteins (Wilkins, J.A. et al., 1996).
Optimal working dilutions must be determined by end user.
质量
Western Blot Analysis:0.5 ?g/mL of the antibody detected Integrin 1 in 10 ?g of U251 cell lysate.
目标描述
88 kDa
外形
Format: Purified
Protein A purified
Purified cultured supernatant in PBS without preservatives.
储存及稳定?/p>
Store at -20C in undiluted aliquots.
Avoid repeated freeze-thaw cycles.
分析说明
Control
Tonsil, human skin, human kidney tissue
U251 cell lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss |
32160702 |
| NACRES |
NA.41 |
产品性质
| 质量水平 |
100 |
| 生物来源 |
mouse |
| 抗体形式 |
culture supernatant |
| antibody product type |
primary antibodies |
| 克隆 |
N29, monoclonal |
| species reactivity |
human |
| manufacturer/tradename |
Chemicon? |
| technique(s) |
cell culture | mammalian: suitable
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable |
| 同位?亚型 |
IgG |
| NCBI登记叶/td> |
NM_002292.3 |
| UniProt登记叶/td> |
P55268 |
| 运输 |
wet ice |
安全信息
| 储存分类代码 |
12 - Non Combustible Liquids |
| WGK |
WGK 1 |
| 闪点(F) |
Not applicable |
| 闪点(C) |
Not applicable |
m.cnreagent.com
