产品介绍９/div>
产品说明
一般描?/p>
Integrin alpha-5 (UniProt P11688; CD49 antigen-like family member E, CD49e, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5) and Integrin beta-1 (UniProt P09055; CD29, Fibronectin receptor subunit beta, GpIIa, VLA-4 subunit beta) are encoded by the Itga5 (VLA5, Fnra; Gene ID 16402) and the Itgb1 (Fnrb; Gene ID 16412) gene, respectively, in murine species. The integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor. The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented. The adhesion receptor alphaVbeta1 is also known as VLA-5 (very late antigen-5) and the primary receptor for fibronectin. VLA-5 is reported to be involved many important physiological events, including angiogenesis, inflammation, neural crest development and cardiovascular morphogenesis.
免疫厞/p>
SSC-VII cells.
应用
This Anti-Integrin 51 Antibody, clone BMA5 is validated for use in Flow Cytometry, Immunoprecipitation, Affects Function for the detection of Integrin 51.
Immunoprecipitation Analysis: A representative lot immunoprecipitated integrin VLA-5 (V1) complex from murine cells, including SSC-VII oral cavity cancer cells, B16F1 melanoma cells, and mast cells (CFTL-15 & MC/9, BMMCs) (Fehlner-Gardiner, C.C., et al. (1996). Differentiation. 60(5):317-325).
Affects Function: A representative lot suppressed 2GPI surface expression on RAW264.7 cells and attenuated cardiolipin-2GPI (aCL/2GPI) complex- and gelsolin-mediated cellular p38 and FAK phosphorylation (Bohgaki, M., et al. (2011). J. Cell. Mol. Med. 15(1):141-151).
Affects Function: A representative lot, when combined with anti-Integrin 3 antibody, greatly blocked EphA8-stimulated NIH/3T3 adhesion to fibronectin surface (Gu, C., and Park, S. (2001). Mol Cell Biol. 21(14):4579-4597).
Affects Function: A representative lot, when combined with anti-Integrin 4, clone PS/2 (Cat. No. CBL1304), greatly blocked PMA-stimulated adhesion of spleen T-cells from non-obese diabetic (NOD) mice to fibronectin-coated surface (Uniyal, S., et al. (1999). J. Autoimmun. 12(3):167-176).
Affects Function: A representative lot, when administered together with anti-Integrin 4, clone PS/2 (Cat. No. CBL1304) via intraperitoneal injection, greatly reduced pancreas islet leukocyte infiltration among non-obese diabetic (NOD) mice following diabetes onset induction by cyclophosphamide injection (Uniyal, S., et al. (1999). J. Autoimmun. 12(3):167-176).
Affects Function: A representative lot blocked integrin V1-mediated adhesion of murine bone marrow-derived mast cells (BMMCs) to fibronectin-coated surface upon PMA stimulation (Fehlner-Gardiner, C.C., et al. (1996). Differentiation. 60(5):317-325).
Flow Cytometry Analysis: A representative lot detected integrin VLA-5 (V1) immunoreactivity on the surface of murine peritoneal macrophages and peripheral blood monocytes (Kapetanovic, R., et al. (2011). Am. J. Physiol. Cell Physiol. 300(4):C850-859).
Flow Cytometry Analysis: A representative lot detected integrin VLA-5 (V1) immunoreactivity on the surface of murine embryonic fibroblasts (Gupta, S.K., and Vlahakis, N.E. (2009). J. Cell Sci. 122(Pt 12):2043-2054).
Flow Cytometry Analysis: A representative lot detected integrin VLA-5 (V1) immunoreactivity on the surface of CD4+ or CD8+ T-cells from peripheral blood and spleen of non-obese diabetic (NOD) mice (Uniyal, S., et al. (1999). J. Autoimmun. 12(3):167-176).
Flow Cytometry Analysis: A representative lot detected integrin VLA-5 (V1) immunoreactivity on the surface of murine bone marrow-derived mast cells (BMMCs) (Fehlner-Gardiner, C.C., et al. (1996). Differentiation. 60(5):317-325).
质量
Evaluated by Flow Cytometry in NIH/3T3 cells.
Flow Cytometry Analysis: 100 ?L of 1:200 dilution from this antibody detected Integrin 51 in NIH/3T3 cells.
目标描述
115 kDa calculated
联系
Replaces: MAB1984
外形
Rat monoclonal IgG2b antibody in tissue culture supernatant without preservatives.
Unpurified
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss |
32160702 |
| NACRES |
NA.41 |
产品性质
| 质量水平 |
100 |
| 生物来源 |
rat |
| 抗体形式 |
culture supernatant |
| antibody product type |
primary antibodies |
| 克隆 |
BMA5, monoclonal |
| species reactivity |
mouse |
| technique(s) |
flow cytometry: suitable
immunoprecipitation (IP): suitable |
| 同位?亚型 |
IgG2b |
| NCBI登记叶/td> |
NP_034707
NP_034708 |
| UniProt登记叶/td> |
P09055
P11688 |
| 运输 |
dry ice |
安全信息
| 储存分类代码 |
10 - Combustible liquids |
| WGK |
WGK 1 |
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