产品介绍９/div>
产品说明
一般描?/p>
14-3-3 protein beta/alpha (KCIP-1, Protein 1054, Protein kinase C inhibitor protein 1), epsilon (14-3-3E), eta (Protein AS1), gamma (KCIP-1, Protein kinase C inhibitor protein 1), theta (14-3-3 protein T-cell, 14-3-3 protein tau, Protein HS1), zeta/delta (KCIP-1, Protein kinase C inhibitor protein 1) are encoded by the YWHAB, YWHAE, YWHAH, YWHAG, YWHAQ, YWHAZ gene (Gene ID 7529, 7531, 7533, 7532, 10971, 7534, respectively) in human. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers, thus rendering 14-3-3 as a key multifunctional regulatory molecule. 14-3-3 isomers have been implicated in several neurological disorders: for example isoforms gamma, epsilon and beta are expressed in the cerebrospinal fluid of Creutzfeldt-Jakob patients, but not patients suffering from other neurological conditions such as Alzheimers. The 14-3-3 pan antibody is one of a panel of antibodies that were generated against 14-3-3 isoforms and have demonstrated their potential for use as an ELISA-based diagnostic tool. These antibodies will allow the research to investigate the role of this protein in key cellular processes and neurological disorders such as Creutzfeldt-Jakob, Alzheimers and Parkinsons disease.
特异?/p>
This antibody targets the N-terminal end sequences of 14-3-3 alpha/beta (YWHAB), gamma (YWHAG), zeta/delta (YWHAZ), epsilon (YWHAE), eta (YWHAH or YWHA1), and theta (YWHAQ).
免疫厞/p>
A mixture of KLH-conjugated linear peptides corresponding the the N-terminal end sequences of human 14-3-3 alpha/beta (YWHAB), gamma (YWHAG), zeta/delta (YWHAZ), epsilon (YWHAE), eta (YWHAH or YWHA1), and theta (YWHAQ).
应用
Anti-14-3-3 pan, Cat. No. AB9748-I, is a highly specific rabbit polyclonal antibody, that targets 14-3-3 protein and has been tested in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting and .
Immunohistochemistry Analysis: A 1:5,000 dilution from a representative lot detected 14-3-3 immunoreactivity in human prostate cancer, cerebellum, and tonsil tissue sections.
Immunoprecipitation Analysis: 5 ?g from a representative lot immunoprecipitated 14-3-3 from 500 ?g of A431 cell lysate.
Research Category
Neuroscience
质量
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: 0.2 ?g/mL of this antibody detected 14-3-3 in 10 ?g of A431 cell lysate.
目标描述
~28 kDa observed. 28.08/27.85 kDa (human isoform Long/Short; YWHAB), 27.75 kDa (human YWHAZ), 29.17 kDa (human YWHAE), 28.22 kDa (human YWHAH or YWHA1), 28.30 kDa (human YWHAG), 27.76 kDa (human YWHAQ) calculated. Uncharacterized bands may be observed in some lysate(s).
联系
Replaces: AB9748
外形
Affinity purified.
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
储存及稳定?/p>
Stable for 1 year at 2-8C from date of receipt.
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
产品性质
| 质量水平 |
100 |
| 生物来源 |
rabbit |
| 抗体形式 |
affinity isolated antibody |
| antibody product type |
primary antibodies |
| 克隆 |
polyclonal |
| 纯化方式 |
affinity chromatography |
| species reactivity |
human |
| species reactivity (predicted by homology) |
chicken (based on 100% sequence homology),Xenopus(based on 100% sequence homology), fish (based on 100% sequence homology), bovine (based on 100% sequence homology), rat (based on 100% sequence homology), sheep (based on 100% sequence homology), zebrafish (based on 100% sequence homology), rabbit (based on 100% sequence homology), mouse (based on 100% sequence homology), porcine (based on 100% sequence homology) |
| technique(s) |
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable |
| NCBI登记叶/td> |
NP_003395
NP_003396
NP_003397
NP_006752
NP_006817
NP_036611 |
| UniProt登记叶/td> |
P27348
P31946
P61981
P62258
P63104
Q04917 |
| 运输 |
ambient |
| Gene Information |
human ... YWHAB(7529), YWHAE(7531), YWHAG(7532), YWHAH(7533), YWHAQ(10971), YWHAZ(7534) |
安全信息
| 储存分类代码 |
12 - Non Combustible Liquids |
| WGK |
WGK 1 |
| 闪点(F) |
Not applicable |
| 闪点(C) |
Not applicable |
m.cnreagent.com
