Anti-phospho SMAD2 Antibody (Ser465/467)

Anti-phospho SMAD2 Antibody (Ser465/467)
Mothers against decapentaplegic homolog 2, Ser465/467 phosphorylated, hMAD-2, Ser465/467 phosphorylated, hSMAD2, Ser465/467 phosphorylated, JV18-1, Ser465/467 phosphorylated, MAD homolog 2, Ser465/467 phosphorylated, Mad-related protein 2, Ser465/467 pho

产品介绍９�/div>

产品说明

一般描�?/p>

Mothers against decapentaplegic homolog 2 (UniProt Q15796; also known as hMAD-2, hSMAD2, JV18-1, MAD homolog 2, Mad-related protein 2, Mothers against DPP homolog 2, SMAD 2, SMAD family member 2, Smad2) is encoded by the SMAD2 (also known as MADH2, MADR2) gene (Gene ID 4087) in human. Smad2 is a receptor-regulated intracellular signal transducer and transcriptional modulator activated by transforming growth factor TGF-beta and activin type 1 receptor kinases. In response to TGF-beta signal, smad2 is recruited to the activated TGF-beta receptor through its interaction with the SMAD anchor for receptor activation (SARA) protein, where it is phosphorylated by the TGF-beta receptor. The phosphorylation results in its dissociation from SARA and association with another family member smad4. The smad2-smad4 complex then translocates into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. Smad2 can also be phosphorylated by activin type 1 receptor kinase, and thereby transduce activin-dependent signaling events.

特异�?/p>

This antibody detects Smad2 Ser465/467 phosphorylation in TGFbeta-stimulated, but not unstimulated HepG2 cells. The corresponding target phosphoserine residues in the alternatively spliced human Smad2Deltaexon3 (Isoform Short) are pSer435 & pSer437 (UniProt Q15796-2).

免疫厞�/p>

KLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Ser465/467 of human Smad2.
Epitope: Phospho-Ser465/467.

应用

Research Sub Category
Transcription Factors
Research Category
Epigenetics & Nuclear Function
Anti-phospho SMAD2 Antibody (Ser465/467) is an antibody against phospho SMAD2 for use in Western Blotting.

质量

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 2.0 ?g/mL of this antibody detected Smad2 Ser465/467 phosphorylation in 10 ?g lysate from TGFbeta-stimulated, but not unstimulated, HepG2 cells.

目标描述

~53 kDa 52.31 kDa (Isoform Long) and 48.96 kDa (Isoform Short; Smad2Deltaexon3) calculated.

联系

Replaces: AB3849

外形

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定�?/p>

Stable for 1 year at 2-8��C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

产品性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	human
species reactivity (predicted by homology)	mouse (based on 100% sequence homology), bovine (based on 100% sequence homology), chicken (based on 100% sequence homology),Xenopus(based on 100% sequence homology), rat (based on 100% sequence homology), zebrafish (based on 100% sequence homology)
technique(s)	western blot: suitable
NCBI登记叶�/td>	NP_005892
UniProt登记叶�/td>	Q15796
运输	wet ice
Gene Information	human ... SMAD2(4087)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable