产品介绍９/div>
产品说明
一般描?/p>
Topoisomerase II (M.W 180 kDa) plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is present ubiquitously in normal cells and is upregulated in tumors and proliferating cells. Topoisomerase II is also implicated in drug resistance of tumor cells.
特异?/p>
In Immunoprecipitation and Western blot experiments the Ki-S1 antibody recognizes a major protein of 170 kD which was identified as the a isoform of topoisomerase II (Boege et al., 1995; Kreipe et al., 1993). In addition, it has been shown that the Ki-S1 antibody recognizes a carboxyterminal a-isoenzyme specific epitope missing in topoisomerase IIb (Boege et al., 1995). In immunohistochemistry the Ki-S1 antibody shows strong nuclear staining only in proliferating cells. The epitope recognized by the antibody is also detectable in paraffin-embedded tissue sections (Kreipe et al., 1993). Accordingly, it has been shown that the expression of topoisomerase IIa is strongly restricted to proliferating cells (Tsutsui et al., 1993). The topoisomerase IIa antigen is preferentially expressed during G 1 , S, G 2 and M phase of the cell cycle, while resting, non-cycling cells (G 0 phase) lack topoisomerase IIa. In addition, constantly proliferating cells (e.g. cell lines) react positively to topoisomerase IIa during the entire cell-cycle. This specificity of Ki-S1 antibody for proliferating cells might make it a useful tool for determination of the proliferative fraction in solid tumors such as mammary carcinomas (Kreipe et al., 1993; Sampson et al., 1992; Camplejohn et al., 1993; Kreipe et al., 1993; Rasbridge et al., 1994) and gangliomas (Wolf et al., 1994).
应用
Anti-Topoisomerase II Antibody, clone KiS1 is a Mouse Monoclonal Antibody for detection of Topoisomerase II also known as Ki-S1 & has been validated in FC, WB, ICC, IHC, IHC(P).
Western blot: 1-10g/ml Immunoprecipitaion
Immunocytochemistry: 5-10 g/ml Immunohistochemistry: 5-10 g/ml
Flow cytometry
Optimal working dilutions must be determined by end user.
联系
Replaces: MABE519
外形
Format: Purified
储存及稳定?/p>
Maintain at 2-8C in undiluted aliquots for up to 6 months.Avoid repeat freeze/thaw cycles.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss |
32160702 |
| NACRES |
NA.41 |
产品性质
| 质量水平 |
100 |
| 生物来源 |
mouse |
| 抗体形式 |
purified immunoglobulin |
| 克隆 |
KiS1, monoclonal |
| species reactivity |
human |
| manufacturer/tradename |
Chemicon? |
| technique(s) |
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable |
| 同位?亚型 |
IgG2a |
| NCBI登记叶/td> |
NM_001067.2 |
| UniProt登记叶/td> |
P11388 |
| 运输 |
wet ice |
| Gene Information |
human ... TOP2A(7153) |
安全信息
| 储存分类代码 |
12 - Non Combustible Liquids |
| WGK |
WGK 2 |
| 闪点(F) |
Not applicable |
| 闪点(C) |
Not applicable |
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