Anti-Stage-Specific Embryonic Antigen-3 Antibody, clone MC-631
Stage-Specific Embryonic Antigen-3
产品介绍９/div>
产品说明
一般描?/p>
Stage-specific embryonic antigen 3 (SSEA-3) is a glycosphingolipid composed of five carbohydrate units connected to a sphingolipid. Sphingolipids are key players in cell signaling and the SSEA-3 is a well known marker of stem cells (ESCs, MSCs, iPSCs, CSCs) differentiation, where SSEA-3 rapidly disappears when the cells start to differentiate.
特异?/p>
Reacts with the Stage-specific embryonic antigen-3 (SSEA-3) that is expressed on the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells. Expression of SSEA-3 is down regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-3 expression.
免疫厞/p>
4-8 cell stage mouse embryos.
应用
This Anti-Stage-Specific Embryonic Antigen-3 Antibody, clone MC-631 is validated for use in Flow Cytometry, Immunocytochemistry, Immunofluorescence and Immunohistochemistry for the detection of Stage-Specific Embryonic Antigen-3.
Research Sub Category
Cell Cycle, DNA Replication & Repair
Immunocytochemistry Analysis: 10?g/mL from a representative lot detected Stage-Specific Embryonic Antigen-3 in H9 cells.
Immunocytochemistry Analysis: A representative lot detected SSEA-3 immunoreactivity among T
cell-derived iPSCs (TiPSCs) by fluorescent immunocytochemistry (Kishino, Y., et al. (2014). PLoS One. 9(5):e97397).
Immunocytochemistry Analysis: A representative lot detected the presence of SSEA-3-positive cancer stem cells (CSCs) among cultured HCT116 colorectal cancer (CRC) cells by fluorescent immunocytochemistry (Suzuki, Y., et al. (2013). Int. J. Oncol. 42(1):161-167).
Immunocytochemistry Analysis: A representative lot detected SSEA-3 immunoreactivity among induced pluripotent stem (iPS) cells from human molars-derived mesenchymal stromal cells (MSCs) by fluorescent immunocytochemistry (Oda, Y., et al. (2010). J. Biol. Chem. 285(38):29270-29278).
Flow Cytometry Analysis: A representative lot detected the presence of SSEA-3-positive cancer stem cells (CSCs) in five cultured colorectal cancer (CRC) cell lines, HCT116, Caco-2, DLD-1, HT-29, and SW480 (Suzuki, Y., et al. (2013). Int. J. Oncol. 42(1):161-167).
Immunofluorescence Analysis: A representative lot detected a mall number of SSEA-3-positive stromal cells in normal colorectal epithelia and SSEA-3-positive cancers in colorectal adenocarcinomas by fluorescent immunohistochemistry using frozen tissue sections (Suzuki, Y., et al. (2013). Int. J. Oncol. 42(1):161-167).
Research Category
Stem Cell Research
质量
Evaluated by Flow Cytometry in H9 cells.
Flow Cytometry Analysis: 1.0 ?g of this antibody detected Stage-Specific Embryonic Antigen-3 in H9 cells.
联系
Replaces: MAB4303
外形
Purified by Ion-Exchange Chromatography
Format: Purified
Purified rat monoclonal IgM antibody in PBS with 0.05% sodium azide.
储存及稳定?/p>
Stable for 1 year at 2-8C from date of receipt.
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
产品性质
| 质量水平 |
100 |
| 生物来源 |
rat |
| 抗体形式 |
purified antibody |
| antibody product type |
primary antibodies |
| 克隆 |
MC-631, monoclonal |
| species reactivity |
human |
| species reactivity (predicted by homology) |
mouse |
| technique(s) |
flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable |
| 同位?亚型 |
IgM |
| 运输 |
wet ice |
安全信息
| 储存分类代码 |
10 - Combustible liquids |
| WGK |
WGK 2 |
| 闪点(F) |
Not applicable |
| 闪点(C) |
Not applicable |
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