产品介绍９/div>
产品说明
一般描?/p>
Recognizes the ~65-68 kDa PKR protein phosphorylated at Thr451.
Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
This PhosphoDetect
Anti-PKR (pThr???) Rabbit pAb is validated for use in Immunoblotting, Immunohistochemistry for the detection of PKR (pThr???).
Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phophorylation site, followed by immunoaffinity chromatography. Recognizes the ~65-68 kDa PKR protein phosphorylated at Thr451.
免疫厞/p>
a synthetic phosphopeptide corresponding to amino acids surrounding the Thr??? phosphorylation site of human PKR (double-stranded RNA-dependent protein kinase)
包装
10 tests in Plastic ampoule
应用
Immunoblotting (1:1000)
Immunohistochemistry (see comments)
警告
Toxicity: Standard Handling (A)
外形
In Dulbeccos PBS (without Mg+2or Ca+2), 0.1% BSA, 50% glycerol, pH 7.3.
重悬
Following initial thaw, aliquot and freeze (-70C).
分析说明
Positive Control
IFN-stimulated HeLa cells.
其他说明
When used at a 1:1000 dilution there is sufficient antibody for 10 blots at 10 ml/blot. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the optimal dilution.
Cuddihy, A.R., et al.1999.Oncogene8,2690.
Srivastava, S.P., et al. 1998.J. Biol. Chem.273,2416.
Clemens, M.J. 1997.Int. J. Biochem. Cell Biol.29,945.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
| 生物来源 |
rabbit |
| 质量水平 |
100 |
| antibody product type |
primary antibodies |
| 克隆 |
polyclonal |
| 形式 |
liquid |
| 包含 |
0.1% sodium azide as preservative |
| species reactivity |
mouse, human |
| manufacturer/tradename |
Calbiochem? |
| 储存条件 |
OK to freeze
avoid repeated freeze/thaw cycles |
| 同位?亚型 |
IgG |
| 运输 |
wet ice |
| 储存温度 |
?70ℂ/td> |
安全信息
| 储存分类代码 |
12 - Non Combustible Liquids |
| WGK |
WGK 2 |
| 闪点(F) |
Not applicable |
| 闪点(C) |
Not applicable |
m.cnreagent.com
