Nomilin 是从柑桔类提取物中获得的柠檬苦素类化合物。Nomilin 是一种抗肥胖症和降糖药、/div>
| 生物活?/td> |
Nomilin is a limonoid compound obtained from the extracts of citrus fruits. Nomilin is an anti-obesity and anti-hyperglycemic agent[1][2].
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体外研究 (In Vitro) |
Nomilin treatment significantly mitigated cell death and decreased lactate dehydrogenase (LDH) release and ROS production in SH-SY5Y cells induced by oxygen-glucose deprivation (OGD), which was almost abolished by Nrf2 knockdown[1].
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体内研究 (In Vivo) |
Nomilin attenuated blood-brain barrier (BBB) disruption in MCAO rats, which might be associated with alleviating the loss of tight junction proteins, including ZO-1 and occludin-5[1].
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| 运输条件 |
Room temperature in continental US; may vary elsewhere.
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| 储存方式 |
4C, protect from light
*In solvent : -80C, 6 months; -20C, 1 month (protect from light)
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| 溶解性数?/td> |
In Vitro:
DMSO : 100 mg/mL(194.34 mM;Need ultrasonic)
配制储备涱/div>
| 1 mM |
1.9434 mL |
9.7170 mL |
19.4341 mL |
| 5 mM |
0.3887 mL |
1.9434 mL |
3.8868 mL |
| 10 mM |
0.1943 mL |
0.9717 mL |
1.9434 mL |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失敇/b>、/span> 储备液的保存方式和期限:-80? 6 months; -20? 1 month (protect from light)、/span>-80 储存时,请在 6 个月内使用,-20 储存时,请在 1 个月内使用、/span>
In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液,再依次添加助溶剂:
——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用 以下溶剂前显示的白br/>分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热?或超声的方式助溶
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1.
请依序添加每种溶剂: 10% DMSO40%PEG3005%Tween-8045% saline
Solubility: 2.5 mg/mL (4.86 mM); Clear solution
此方案可获得 2.5 mg/mL (4.86 mM,饱和度未知) 的澄清溶液、/p>
1 mL 工作液为例, 100 μL 25.0 mg/mL 的澄 DMSO 储备液加 400 μL PEG300 中,混合均匀;向上述体系中加?0 μL Tween-80,混合均匀;然后继续加 450 μL生理盐水定容 1 mL、/p>
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2.
请依序添加每种溶剂: 10% DMSO90%corn oil
Solubility: 2.5 mg/mL (4.86 mM); Clear solution
此方案可获得 2.5 mg/mL (4.86 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验、/p>
1 mL 工作液为例, 100 μL 25.0 mg/mL 的澄 DMSO 储备液加 900 μL玉米油中,混合均匀、/p>
*以上所有助溶剂都可在本网站选购、/span>
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