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Sophoridine

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验、�/div>

CAS NO:

6882-68-4

包装与价格：

包装	价格(�?
10 mM * 1 mL in DMSO	电议
5mg	电议
10mg	电议
20mg	电议
50mg	电议
100mg	电议

产品名称

槐定碰�br />

产品介绍

Sophoridine 是一种从豆科植物苦参中分离出来的喹喔啉生物碱。Sophoridine 诱导细胞凋亡 ( apoptosis)。Sophoridine 有可能成为胰腺癌的有效，选择性和耐受性良好的候选药物、�/div>

生物活�?/td>

Sophoridine is a quinolizidine alkaloid isolated from Leguminous plantSophora flavescens. Sophoridine inducesapoptosis. Sophoridine has the potential to be a novel, potent and selective antitumor drug candidate for pancreaticcancerwith well-tolerated toxicity^[1].

体外研究
(In Vitro)

Sophoridine (0-500 μM; 48 hours) exhibits remarkable inhibition effects to the growth of human pancreatic, gastric, liver, colon, gallbladder, and prostate carcinoma cells with IC₅₀values of about 20 μM to 200 μM^[1].
Sophoridine (0-20 μM; 48 hours) increases S phase cell population from 26.23% (control) to 38.67% in Miapaca-2 cells and from 29.56% (control) to 39.16% in PANC-1 cells, about a 1.5-fold and a 1.3-fold increase, respectively^[1].
Sophoridine (0-20 μM; 48 hours) significantly increases bad and bax levels, and decreases bcl-2 and bcl-xl levels in contrast, with a significant increase in Bax/Bcl-2 ratio^[1].

Cell Viability Assay^[1]

Cell Line:	Normal cells: IOSE144, HL-7702 and LO2, BEAS-2B, GES-1, HEK 293 T, HPDE, FHC, Human cancer cells: PANC-1, Mapaca-1, hepG2, SGC-7901, CBC-SD,SGC-996,PC-3,MKN-45,MGC-803,Hela and HCT116 cells
Concentration:	0, 3.9, 7.8, 15.5, 31, 62.5, 125, 250, 500 μM
Incubation Time:	48 hours
Result:	Exhibited the most potent cytotoxicity to cancer cells.

Cell Cycle Analysis^[1]

Cell Line:	PANC-1 cells; Miapca-2 cells
Concentration:	20 μM
Incubation Time:	48 hours
Result:	Led to accumulated population in the S phase.

Western Blot Analysis^[1]

Cell Line:	PANC-1 cells; Miapca-2 cells
Concentration:	20 μM
Incubation Time:	48 hours
Result:	Induced the activation of intrinsic apoptosis pathway.

体内研究
(In Vivo)

Sophoridine (intraperitoneal injection; 20 or 40 mg/kg; 21 days) can inhibit the growth of xenograft pancreatic tumors^[1].

Animal Model:	BALB/c homozygous (nu/nu) nude mice^[1]
Dosage:	20 or 40 mg/kg
Administration:	Intraperitoneal injection; 20 or 40 mg/kg; 21 days
Result:	Decreased xenograft pancreatic tumors mass.

分子野�/td>

248.36

性状

Solid

Formula

C₁₅H₂₄N₂O

CAS 叶�/td>

6882-68-4

中文名称

槐定碰�/p>

结构分类

Alkaloids
Pyridine Alkaloids

来源

Plants
Leguminosae
Sophora flavescens

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20��C	3 years
	4��C	2 years
In solvent	-80��C	6 months
	-20��C	1 month

溶解性数�?/td>

In Vitro:

DMSO : 50 mg/mL(201.32 mM;Need ultrasonic)

配制储备涱�/div>

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	4.0264 mL	20.1321 mL	40.2641 mL
5 mM	0.8053 mL	4.0264 mL	8.0528 mL
10 mM	0.4026 mL	2.0132 mL	4.0264 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失敇�/b>、�/span>
储备液的保存方式和期限：-80�? 6 months; -20�? 1 month、�/span>-80� 储存时，请在 6 个月内使用，-20� 储存时，请在 1 个月内使用、�/span>

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用� 以下溶剂前显示的白�br/>分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热�?或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO40%PEG3005%Tween-8045% saline

Solubility: � 2.17 mg/mL (8.74 mM); Clear solution

此方案可获得 � 2.17 mg/mL (8.74 mM，饱和度未知) 的澄清溶液、�/p>

� 1 mL 工作液为例，� 100 μL 21.7 mg/mL 的澄� DMSO 储备液加� 400 μL PEG300 中，混合均匀；向上述体系中加�?0 μL Tween-80，混合均匀；然后继续加� 450 μL生理盐水定容� 1 mL、�/p>

� 0.9 g 氯化钠，完全溶解� 100 mL ddH2O 中，得到澄清透明的生理盐水溶涱�/div>
2.

请依序添加每种溶剂： 10% DMSO90% (20%SBE-β-CDin saline)

Solubility: � 2.17 mg/mL (8.74 mM); Clear solution

此方案可获得 � 2.17 mg/mL (8.74 mM，饱和度未知) 的澄清溶液、�/p>

� 1 mL 工作液为例，� 100 μL 21.7 mg/mL 的澄� DMSO 储备液加� 900 μL20% � SBE-β-CD 生理盐水水溶涱�/span>中，混合均匀、�/p>

� 2 g 磺丁基醚 β-环糊精加� 5 mL 生理盐水中，再用生理盐水定容� 10 mL，完全溶解，澄清透明
3.

请依序添加每种溶剂： 10% DMSO90%corn oil

Solubility: � 2.17 mg/mL (8.74 mM); Clear solution

此方案可获得 � 2.17 mg/mL (8.74 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验、�/p>

� 1 mL 工作液为例，� 100 μL 21.7 mg/mL 的澄� DMSO 储备液加� 900 μL玉米油中，混合均匀、�/p>

*以上所有助溶剂都可在本网站选购、�/span>