产品介绍
| 目录叶br/>Serial |
GNM20 |
| 标识?br/>Identifier |
CSTR:19375.09.3101MOUGNM20 |
描述
Description |
MS1?994年建株的胰岛内皮细胞株。原代培养的胰岛内皮细胞用抗G418的温度敏感型SV40大T抗原(tsA-58-3)转染。抗性克隆用克隆环分离，并筛选吸收dil-Ac-LDL的。这株细胞保留了内皮细胞的许多特性，如吸收乙 酰化LDL和表达八因子相关抗原及BEGF受体、br/>在本库通过支原体检测、/span> |
动物种别
Organism |
小鼠 |
性别
Gender |
*** |
组织来源
Tissue and Cell Type |
正常胰岛内皮，SV40转染 |
| 形?br/>Morphology |
*** |
| 培养基和添加剁br/>Complete Growth Medium and Culture Conditions |
DMEM培养?GIBCO by life technologies ,货号C11995500BT)?5%；优质胎牛血清，5%、br/>气相：空气，95%；二氧化碳，5%、br/>温度?7摄氏度、/span> |
供应限制
Permits and Restrictions |
A。仅供研究之用、/span> |
| REFERENCE |
Arbiser JL , et al. Oncogenic H-ras stimulates tumor angiogenesis by two distinct pathways. Proc. Natl. Acad. Sci. USA 94: 861-866, 1997. PubMed: 9023347Arbiser JL , et al. Overexpression of VEGF 121 in immortalized endothelial cells causes conversion to slowly growing angiosarcoma and high level expression of the VEGF receptors VEGFR-1 and VEGFR-2 in vivo. Am. J. Pathol. 156: 1469-1476, 2000. PubMed: 10751370Hay, R. J., Caputo, J. L., and Macy, M. L. Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.Centers for Disease Control (1993), Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S. Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C. |
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