| 目录叶br/>Serial |
SCSP-213 |
| 标识?br/>Identifier |
CSTR:19375.09.3101MOUSCSP213 |
描述
Description |
带Oct4-Neo基因的小鼠全能性胚胎干细胞，每支细胞量1×106。每个批次均通过本库支原体检测，结果为阴性、/span> |
动物种别
Organism |
小鼠，C57BL/6J×C57BL/6J品系 |
性别
Gender |
|
组织来源
Tissue and Cell Type |
小鼠胚胎内细胞团 |
| 形?br/>Morphology |
球形克隆 |
| 培养基和添加剁br/>Complete Growth Medium and Culture Conditions |
mES完全培养液配?600 ml)９br/>DMEM (Invitrogen 12430) 497 ml
ES级FBS (biochrom S4115) 90 ml
Glutamax (Invitrogen 35050) 6 ml
NEAA (Invitrogen 11140) 6 ml
LIF (Millipore ESG1107) 60 μl(终浓度为1000U/ml)
β-Mer (Invitrogen 21985) 1.5 ml
气相：空气，95%；二氧化碳，5%。温度：37摄氏度、/span> |
供应限制
Permits and Restrictions |
A。仅供研究之用、/span> |
| REFERENCE |
Chen et al., Self-renewal of embryonic stem cells by a small molecule. PNAS, 103.No. 46. (2006), 17266?7271 |
