| 目录叶br/>Serial |
SCSP-204 |
| 标识?br/>Identifier |
CSTR:19375.09.3101MOUSCSP204 |
描述
Description |
该细胞缺乏HGPRT(HPRT)，并且对0.06 mM?-硫鸟嘌呤有抗性。当在饲养层(胚胎成纤维细胞或STO细胞)上培养时，细胞保持未分化状态。在没有饲养层的情况下，细胞自发分化并形成胚胎结构。当注入胚泡中时，细胞可以进入生殖系。在常规的分子基因修饰技术之后，它们可用于重构小鼠胚胎。每个批次均通过本库支原体检测，结果为阴性。我库冻存时为P8代、br/>培养时需使用昆明白MEF(SCSP-101R)作为饲养层细胞、/span> |
动物种别
Organism |
小鼠?29/Ola品系 |
性别
Gender |
|
组织来源
Tissue and Cell Type |
小鼠胚胎内细胞团 |
| 形?br/>Morphology |
球形，贴壁生镾/span> |
| 培养基和添加剁br/>Complete Growth Medium and Culture Conditions |
mES完全培养液配?600 ml)９br/>DMEM(Invitrogen 12430054)497 ml
ES级FBS (Gibco) 90 ml
Glutamax(Invitrogen 35050061) 6 ml
NEAA(Invitrogen 11140050) 6 ml
LIF(Millipore ESG1107) 60 μl(终浓度为1000U/ml)
β-Mer(Invitrogen 21985) 1.5 ml
气相：空气，95%；二氧化碳，5%。温度：37摄氏度、/span> |
供应限制
Permits and Restrictions |
A。仅供研究之用、/span> |
| REFERENCE |
Smith AG, Hooper ML. Buffalo rat liver cells produce a diffusible activity which inhibits the differentiation of murine embryonal carcinoma and embryonic stem cells. Dev. Biol. 121: 1-9, 1987. PubMed: 3569655Kuehn MR, et al. A potential animal model for Lesch-Nyhan syndrome through introduction of HPRT mutations into mice. Nature 326: 295-298, 1987. PubMed: 3029599Doetschman T, et al. Targetted correction of a mutant HPRT gene in mouse embryonic stem cells. Nature 330: 576-578, 1987. PubMed: 3683574 |
