产品介绍
| 目录叶br/>Serial |
SCSP-222 |
| 标识?br/>Identifier |
CSTR:19375.09.3101MOUSCSP222 |
描述
Description |
通过将绿色荧光蛋?GFP)插入到Olig2基因(一种谱系特异性的转录因子)中，构建获得G-Olig2细胞。在该细胞神经分化的活细胞中，可以观察到GFP表达。每个批次均通过本库支原体检测，结果为阴性、/span> |
动物种别
Organism |
小鼠?29X1/SvJ品系 |
性别
Gender |
|
组织来源
Tissue and Cell Type |
小鼠胚胎内细胞团 |
| 形?br/>Morphology |
球形克隆 |
| 培养基和添加剁br/>Complete Growth Medium and Culture Conditions |
mES完全培养液配?600 ml)９br/>DMEM (Invitrogen 12430) 497 ml
ES级FBS (biochrom S4115) 90 ml
Glutamax (Invitrogen 35050) 6 ml
NEAA (Invitrogen 11140) 6 ml
LIF (Millipore ESG1107) 60 μl(终浓度为1000U/ml)
β-Mer (Invitrogen 21985) 1.5 ml
气相：空气，95%；二氧化碳，5%。温度：37摄氏度、/span> |
供应限制
Permits and Restrictions |
A。仅供研究之用、/span> |
| REFERENCE |
Matise M, et al., Production of targeted embryonic stem cell clones. In: Matise M, et al., Gene Targeting: A Practical Approach, Oxford, Oxford University Press, 101-132, 1999Xian HQ, et al. A subset of ES-cell-derived neural cells marked by gene targeting. Stem Cells 21: 41-49, 2003. PubMed: 12529550 |
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