产品介绍
| 目录叶br/>Serial |
SCSP-550 |
| 标识?br/>Identifier |
CSTR:19375.09.3101MOUSCSP550 |
描述
Description |
通过电镜观察，这些细胞表现出典型的肝细胞特征，如过氧化物酶体和胆管状结构。AML12(小鼠?2)细胞系是从携带人TGF - alpha基因的转基因小鼠(CD1株，MT42?的肝细胞中构建的。AML12细胞保留了表达血?白蛋白、? 1抗胰蛋白酶和转铁蛋白)和缝隙连?连接蛋白26?2)的高水平mRNA的能力，并且只包含乳酸脱氢酶的同工酶5、br/>细胞质控结果９br/>1.支原体检测结果：每个批次均通过本库支原体检测，结果为阴性、br/>2.STR鉴定结果９br/>①该株细胞DNA进行小鼠细胞STR分型结果显示，扩增后图谱清晰，分型结果良好：1-1?1.1?-2?3?-1??-2?2?-2?0.3?-5?4, 15?-4?5.3?-7?2?-1?9?-1?4, 15?1-2?8?2-1?9?3-1?5.1?5-3?1.3?7-2?3, 14, 15?8-3?1?9-2?3；X-1?6、br/>②该株细胞确为小鼠细胞，没有人源细胞污染、/span> |
动物种别
Organism |
小鼠，CD1品系?月龄 |
性别
Gender |
雄?/span> |
组织来源
Tissue and Cell Type |
肝脏 |
| 形?br/>Morphology |
上皮细胞 |
| 培养基和添加剁br/>Complete Growth Medium and Culture Conditions |
小鼠肝实质细胞AML12完全培养液配?100 mL)９br/>DMEM/F-12(1:1) (Invitrogen, 11330-032) 89 ml
ITS Liquid Media Supplement (Sigma, I3146) 1 ml
Dexamethasone 40 ng/ml
FBS (Gibco) 10 ml
参考传代周期：?-3天传一次代
参考传代比例：1:4-1:6
参考换液频率：每周2-3欠br/>冻存液：完全培养?5%，DMSO 5%
细胞状态：贴壁生长
气相：空气，95%；二氧化碳，5%。温度：37摄氏度、/span> |
供应限制
Permits and Restrictions |
A。仅供研究之用、/span> |
| REFERENCE |
Wu JC, et al. Establishment and characterization of differentiated, nontransformed hepatocyte cell lines derived from mice transgenic for transforming growth factor alpha. Proc. Natl. Acad. Sci. USA 91: 674-678, 1994. PubMed: 7904757Dumenco L, et al. Introduction of a murine p53 mutation corresponding to human codon 249 into a murine hepatocyte cell line results in growth advantage, but not in transformation. Hepatology 22: 1279-1288, 1995. PubMed: 7557882 |
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