B6/BLU小鼠胚胎干细胝�/title> - �뵺�羺�ٷ��ַ

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B6/BLU小鼠胚胎干细胝�/div>

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产品介绍

目录叶�br/>Serial	SCSP-226
标识�?br/>Identifier	CSTR:19375.09.3101MOUSCSP226
描述 Description	该细胞系被报道具有生殖能力，源自一个携带LacZ报告基因的C57BL/6转基因小鼠。这段转基因在pUC19中组装，包含1.9 kb�?4 HS-2片段(两侧KpnI/PvuII酶切位点)，位于标记的?-珠蛋白转基因上游。每个批次均通过本库支原体检测，结果为阴性、�br/>培养时需使用昆明白MEF(SCSP-101R)作为饲养层细胞、�/span>
动物种别 Organism	小鼠；C57BL/6Tac品系
性别 Gender	雃�/span>
组织来源 Tissue and Cell Type	内细胞团
形�?br/>Morphology	球形克隆
培养基和添加剁�br/>Complete Growth Medium and Culture Conditions	mES完全培养液配�?600 ml)９�br/>DMEM (Invitrogen 12430) 497 ml ES级FBS (Gibco) 90 ml Glutamax (Invitrogen 35050-061) 6 ml NEAA (Invitrogen 11140-050) 6 ml LIF (Millipore ESG1107) 60 μl(终浓度为1000U/ml) β-Mer (Invitrogen 21985) 1.5 ml 气相：空气，95%；二氧化碳，5%。温度：37摄氏度、�/span>
供应限制 Permits and Restrictions	A。仅供研究之用、�/span>
REFERENCE	Hughes, DE et al. Genetic variation in C57BL/6 ES cell line. Mamm. Genome 18: 549-558, 2007. PubMed: 17828574Ware, BC, et al. Utiltiy of a C57BL/6 ES line versus 129 ES lines for targeted mutations in mice. Transgenic Res. 12: 743-746, 2003. PubMed: 14713204Graubert A T, et al. Stochastic, stage-specific mechanisms account for the variegation of a human globin transgene. Nucleic Acids Research. 26 (12) :2849-2858, 1998. PubMed: 9611227