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Apostatin-1
本半岛bd体育手机客户端 不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
Apostatin-1图片
CAS NO: 2559703-06-7
包装与价格：
包装 价格(元)
10 mM * 1 mL in DMSO 电议
5mg 电议
10mg 电议
25mg 电议
50mg 电议
100mg 电议
200mg 电议
500mg 电议

半岛bd体育手机客户端 名称
Apt-1
半岛bd体育手机客户端 介绍
Apostatin-1 (Apt-1) 是一种有效的 TRADD抑制剂。Apostatin-1 可与 TRADD-N结合 ( KD=2.17 μM)，破坏其与 TRADD-C 和 TRAF2 的结合。Apostatin-1 调节 RIPK1和 beclin 1的泛素化。Apostatin-1 通过激活突变 tau、α-synuclein 或 huntingtin 积累的细胞中的自噬 ( autophagy)，阻断凋亡 ( apoptosis)，并恢复细胞内稳态。
生物活性

Apostatin-1 (Apt-1) is a potentTRADDinhibitor. Apostatin-1 can bind withTRADD-N(KD=2.17 μM), disrupting its binding to both TRADD-C and TRAF2. Apostatin-1 modulates the ubiquitination ofRIPK1andbeclin 1. Apostatin-1 blocksapoptosisand restores cellular homeostasis by activatingautophagyin cells with accumulated mutant tau,α-synuclein, orhuntingtin[1].
体外研究
(In Vitro)

Apostatin-1 inhibits Velcade (Bortezomib, HY-134050)-induced apoptosis and RIPK1-dependent apoptosis (RDA) and necroptosis, with an IC50of 0.97 μM[1].
Apostatin-1 (10 μM, 6 h) effectively induces autophagy and the degradation of long-lived proteins[1].

Western Blot Analysis[1]

Cell Line: SH-SY5Y, HeLa, HT-29, and Jurkat cells
Concentration: 10 μM
Incubation Time: 6 h
Result: Effectively induced autophagy by LC3 II induction and p62 reduction.
体内研究
(In Vivo)

Apostatin-1 (20 mg/kg, IP, once) inhibits inflammatory responses, and increases survival of systemic inflammation mouse model[1].

Animal Model: Wild-type mice (n = 10, male, 8 weeks of age, systemic inflammation mouse model)[1]
Dosage: 20 mg/kg
Administration: IP, once
Result: Reduced expression of the TNF-induced inflammatory target gene products, NOS and COXII27, and of inflammatory cytokines in cells stimulated with pathogen-associated molecular patterns, including interferon γ (IFNγ), lipopolysaccharide (LPS), Pam3CSK4 (a synthetic bacterial lipopeptide), and muramyl dipeptide (MDP). Showed increased survival following intravenous delivery of TNF, a mouse model of systemic inflammation.
分子量

345.50
性状

Solid
Formula

C19H27N3OS
CAS 号

2559703-06-7
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro:

DMSO : 83.33 mg/mL(241.19 mM;ultrasonic and warming and adjust pH to 4 with HCl and heat to 80℃)

配制储备液
浓度溶剂体积质量 1 mg 5 mg 10 mg
1 mM 2.8944 mL 14.4718 mL 28.9436 mL
5 mM 0.5789 mL 2.8944 mL 5.7887 mL
10 mM 0.2894 mL 1.4472 mL 2.8944 mL
*

请根据半岛bd体育手机客户端 在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的半岛bd体育手机客户端 失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。
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