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WST-1
本半岛bd体育手机客户端 不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
WST-1图片
CAS NO: 150849-52-8
包装与价格:
包装 价格(元)
10mM (in 1mL DMSO) 电议
10mg 电议
25mg 电议
50mg 电议
100mg 电议

半岛bd体育手机客户端 介绍
A water-soluble and cell-permeable tetrazolium dye
Cas No. 150849-52-8
别名 2-(4-碘苯)-3-(4-硝基苯)-5-(2,4-二磺基苯)-2H-四氮唑钠盐
Canonical SMILES [O-]S(C(C=C1)=CC(S([O-])(=O)=O)=C1C2=NN(C3=CC=C(I)C=C3)[N+](C4=CC=C([N+]([O-])=O)C=C4)=N2)(=O)=O.[Na+]
分子式 C19H11IN5O8S2?Na
分子量 651.3
溶解度 DMF: 5 mg/ml,DMSO: 10 mg/ml,PBS (pH 7.2): 10 mg/ml
储存条件 -20°C
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
半岛bd体育手机客户端 描述

WST-1 is a water-soluble and cell-permeable tetrazolium dye.1,2,3Upon NADH-dependent enzymatic cleavage by cellular mitochondrial dehydrogenases, formazan is released, which can be quantified by colorimetric detection at 450 nm as a measure of cell viability.

1.Francoeur, A.M., and Assalian, A.Microcat: A novel cell proliferation and cytotoxicity assay based on WST-1Biochemica319-25(1996) 2.Ishiyama, M., Shiga, M., Sasamoto, K., et al.A new sulfonated tetrazolium salt that produces a highly water-soluble formazan dyeChem. Pharm. Bull.41(6)1118-1122(1993) 3.Ishiyama, M., Tominaga, H., Shiga, M., et al.A combined assay of cell viability and in vitro cytotoxicity with a highly water-soluble tetrazolium salt, neutral red and crystal violetBiol. Pharm. Bull.19(11)1518-1520(1996)

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