| 订货叶/td> | 纯度规格 | 包装 | 价格(? |
| 173826 | BioReagent, for molecular biology, powder | 1G | - |
| 173828 | BioReagent, for molecular biology, powder | 5G | - |
| 173827 | BioReagent, for molecular biology, powder | 25G | - |
| 品牌: | Sigma |
| 等级规格: | BioReagent, for molecular biology, powder |
| 交货朞 | 1??/td> |
| 产地: | 进口 |
| CAS NO: | 1239-45-8 |
| 产品别名: | 3,8-二氨?5-乙基-6-苯基菲啶溴化?br />胡米溴铵 Ethidium bromide 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide EtBr Homidium bromide |
贝尔斯坦登记号【Beilstein Registry Number : 3642536
欧洲现有商业化学品目录编号【EC Number : 214-984-6
MDL编号(化学反应和变化识别号)【MDL number : MFCD00011724
PubChem数据库物质ID【PubChem Substance ID : 24894653
生物化学/生理学作用【Biochem/physiol Actions :
溴化乙啶可插入双 DNA RNA 中,并作为移码诱变剂。它还可与吖啶橙结合用于区分存活的、凋亡的和坏死的细胞
包装【Packaging : 1, 5, 25 g in glass bottle
配制【Reconstitution :
对于在电泳后进行凝胶染色,应先用水将储存液样品稀释到 0.5μg/ml,再将凝胶孵 15-30 分钟。通常无需脱色,但是如果必须降低背景色,则可在?水中脱色 15 分钟。然后可在紫外灯箱(254nm 波长)中检 DNA 条带。也可将溴化乙啶加到凝胶和电泳缓冲液(终浓度 0.5μg/ml)中,并在电泳后立即显影、br/> 应用【Application :
Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA.4 Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.
Frameshift mutagen which intercalates double-stranded DNA and RNA.
等级【grade : for molecular biology
产品线【product line : BioReagent
形态【form : powder
熔点【mp : 260-262 (dec.)(lit.)
适用性【suitability : suitable for gel electrophoresis
符号【Symbol : GHS06, GHS08
信号词【Signal word : Danger
危害声明【Hazard statements : H302-H330-H341
警示性声明【Precautionary statements : P260-P281-P284-P310
个人防护装备【Personal Protective Equipment : Eyeshields, Faceshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges
危害?欧洲)【Hazard Codes (Europe) : T+
风险声明(欧洲)【Risk Statements (Europe) : 22-26-68
安全声明(欧洲)【Safety Statements (Europe) : 28-36/37-45-63
欧洲内陆运输危险品【RIDADR : UN 2811PIH 6.1 / PGI
德国水质危险性分类【WGK Germany : 3
化学物质毒性作用登记号【RTECS : SF7950000
分子结构式: