Trizma^® 盐酸盐 盐酸盐

NH₂C(CH₂OH)₃ · HCl

157.60

3675235

MFCD00012590

Tris HCl is a commonly used biological buffer. Tris/Tris HCl as a buffering system has a very broad usage in biological systems for biopharmaceutical manufacturing, cell culture, diagnostics and molecular biology. The pKa of Tris is 7.77 which corresponds closely to a physiological pH of 7.36.

Tris HCl is used as the conjugate buffer component with Tris/Tromethamine/Trizma in biopharmaceutical buffer formulations (both upstream and downstream applications), electrophoresis separations and diagnostic reagent formulations.
所有缓冲液的pH值都与温度和浓度有关。对于Tris缓冲液，降温每℃其pH值约增加0.03单位，每10倍稀释其pH降低0.03-0.05单位。
对于精确的应用，请使用带有玻璃/甘汞组合电极并仔细校准过的pH计。

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany
Trizma is a registered trademark of sigma-aldrich Co. LLC

400

synthetic

EMPROVE^® EVOLVE

powder

cell culture | mammalian: suitable

Endotoxin, tested
Microbial, tested
Trace Metals, tested

7.0-9.0

8.1

suitable for (Suitable for use as a raw material in bioprocessing)
suitable for manufacturing use

Cytotoxicity, contains (tested)
DNase, contains (tested)
NICKase, contains (tested)
Protease, contains (tested)
RNase, contains (tested)

Cl.NC(CO)(CO)CO

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

QKNYBSVHEMOAJP-UHFFFAOYSA-N

11 - Combustible Solids

WGK 1