| 货号:26903 |
| 规格:30 μL |
| 基因符号:RAC1 |
| 描述:Anti-Rac1-GTP Monoclonal Antibody |
| 背景介绍:Small GTPases are a super-family of cellular signaling regulators. Rac belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases the activity of Rac, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). |
| 免疫厞Recombinant full length protein of active Rac1 |
| 经过测试的应?IP, IHC, IF |
| 推荐稀释比:IP: 1 μg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
| 浓度:1 mg/ml |
| 宿主:Mouse |
| 形式:Liquid |
| 克隆?Monoclonal |
| 亚型:IgM |
| 纯度:Purified from ascites |
| 防腐剁No |
| 成分:PBS (without Mg2+and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应?Anti-Rac1-GTP monoclonal antibody recognizes active Rac1 from vertebrates. |
| 储存条件:Store at -20°C. Avoid repeated freezing and thawing |
免疫沉淀/蛋白质印迸 Rac Activation Assay.MEF cells were treated with (lane 2) or without (lane 1) PDGF. Cell lysates were incubated with an anti-Rac-GTP monoclonal antibody (Cat. # 26903) (top panel). Th precipitated active Rac was immunoblotted with an anti-Rac rabbit polyclonal antibody (Cat. # 21003). The bottom panel shows the Western blot with anti-Rac of the cell lysates used (5% of that used in the top panel). |
免疫荧光: Immunofluorescence for the active Rac using Anti-Rac1-GTP Mouse Monoclonal Antibody [26903] shows Rac-GTP immunolabeling (green) in combination with cofilin (red) on brain tissue sections. The tissue sections were fixed with -20 oC methanol or 4% paraformaldehyde (fixation time 1 hr) and stained with antibody at 1: 1000 in 0.1M Phosphate buffer with 0.3% Triton X, and 4% BSA for 24h at room temperature. Secondary antibodies were anti-mouse AlexaFluor488 and anti-rabbit AlexaFluor594 at 1: 1000. |
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Immunofluorescence for the active Rac using Anti-Rac1-GTP Mouse Monoclonal Antibody [26903] shows Rac-GTP immunolabeling (green) in combination with cofilin (red) on brain tissue sections. The tissue sections were fixed with -20 oC methanol or 4% paraformaldehyde (fixation time 1 hr) and stained with antibody at 1: 1000 in 0.1M Phosphate buffer with 0.3% Triton X, and 4% BSA for 24h at room temperature. Secondary antibodies were anti-mouse AlexaFluor488 and anti-rabbit AlexaFluor594 at 1: 1000.
