Biotin-16-dCTP,orb64049
| 型号: |
orb64049 |
| 品牌: |
biorbyt |
| 参考价栻 |
6539?mL |
| 纯度含量: |
95% (HPLC) |
| 等级规格: |
特级 |
| 包装: |
1ml |
| 交货朞 |
2~4?/td> |
| 产地: |
UK |
| 分子弎 |
C32H51N8O17P3S |
| 分子野 |
Theoretical MW: 944.78 g/mol (free acid); Detected |
产品名称
Biotin-16-dCTP(biotin)
产品介绍
| Catalog Number |
orb64049 |
| Category |
Tools |
| Description |
Biotin-16-dCTP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detec-tion of the Biotin moiety is ensured by a 16-atom linker attached to the C5 position of cytidine. Recommended Biotin-16-dCTP/dCTP ratio for PCR: 50% Biotin-16-dCTP/50% dCTP. |
| Concentration |
1.0 mM-1.1 mM |
| Form/Appearance |
filtered solution (30 kDa) in 10 mM Tris-HCl; Color: colorless to slightly yellow; pH: 7.5 ± 0.5 |
| Purity |
95% (HPLC) |
| MW |
Theoretical MW: 944.78 g/mol (free acid); Detected MW: 944.23 g/mol (free acid) |
| SMILES |
C1[C@H]2[C@@H]([C@@H](S1)CCCCC(=O)NCCCCCC(=O)NCCCC(=O)NCC#Cc1cn(c(=O)nc1N)[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)C1)NC(=O)N2 |
| Formula |
C32H51N8O17P3S |
| Storage |
store at -20 °C |
| Note |
For research use only |
| Application notes |
Incorporation into DNA/cDNA by: PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase I; Primer Extension with Klenow exo-; 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) ; Reverse Transcription with MMLV Reverse Transcriptase.
Spectroscopic Propertie: λmax 294 nm, ε 9.3 L mmol-1 cm-1 (Tris-HCl, pH 7.5). |
| Expiration Date |
12 months from date of receipt. |
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