产品介绍
| 目录叶br/>Serial |
GNHu41 |
| 标识?br/>Identifier |
CSTR:19375.09.3101HUMGNHu41 |
描述
Description |
MRC-5细胞系来?4周龄男性胎儿的正常肺组织，该细胞老化前能传代42?6次群体倍增 它是正常二倍体细胞系，46，XY核型 模式染色体数?6，概率为70%、br/>在本库通过支原体检测、br/>在本库通过STR检测、/span> |
动物种别
Organism |
亹/span> |
性别
Gender |
甶/span> |
组织来源
Tissue and Cell Type |
肹/span> |
| 形?br/>Morphology |
成纤维细胝/span> |
| 培养基和添加剁br/>Complete Growth Medium and Culture Conditions |
MEM培养?GIBCO,货号41500034，添加NaHCO3 1.5g/L，Sodium Pyruvate 0.11g/L)?0%；优质胎牛血清，10%、br/>气相：空气，95%；二氧化碳，5%、br/>温度?7摄氏度、/span> |
供应限制
Permits and Restrictions |
A。仅供研究之用、/span> |
| REFERENCE |
Jacobs JP , et al. Characteristics of a human diploid cell designated MRC-5. Nature 227: 168-170, 1970. PubMed: 4316953Proc. Symp. Human Diploid Cells. Zagreb, Yugoslavia: Yugoslav Acad. Sci. Arts; 1970, pp. 43-55.Holliday R , Tarrant GM . Altered enzymes in ageing human fibroblasts. Nature 238: 26-30, 1972. PubMed: 12635262Lewis CM , Tarrant GM . Error theory and ageing in human diploid fibroblasts. Nature 239: 316-318, 1972. PubMed: 12635218Landers JE , et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029Kaur A , et al. Cytotoxic T-lymphocyte responses to cytomegalovirus in normal and simian immunodeficiency virus-infected Rhesus macaques. J. Virol. 70: 7725-7733, 1996. PubMed: 8892893MOLECULAR THERAPY 11(4):531,2005 |
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