A-375 [A375]人恶性黑色素瘤细�?2013年新引进)

细胞培养� >> 细胞�

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目录叶�br/>Serial	SCSP-533
标识�?br/>Identifier	CSTR:19375.09.3101HUMSCSP533
描述 Description	人恶性黑色素瘤细胞A-375 [A375]来源于一�?4岁的女性。在裸鼠中有致瘤性。每个批次均通过本库支原体检测，结果为阴性。经本库STR检测结果无误。STR结果如下：D5S818: 12；D13S317: 11,14；D7S820:9；D16S539: 9；vWA: 16,17；THO1: 8；Amelogenin: X；TPOX: 8,10；CSF1PO: 11,12
动物种别 Organism	亹�/span>
性别 Gender	女性，54�?/span>
组织来源 Tissue and Cell Type	皮肤
形�?br/>Morphology	上皮样，贴壁生长
培养基和添加剁�br/>Complete Growth Medium and Culture Conditions	人皮肤黑色素瘤细胞A-375[A375]细胞完全培养液配�?100 ml)９�br/>DMEM (Invitrogen, 11960044)88 ml FBS (Gibco) 10 ml Glutamax (Invitrogen, 35050061) 1 ml Sodium Pyruvate (Invitrogen, 11360-070) 1 ml(终浓�? mM) 参考传代周期：2-3�?br/>参考传代比例：1:3-1:5 参考换液频率：2-3�?br/>冻存液：完全培养�?5%，DMSO 5% 细胞状态：贴壁生长气相：空气，95%；二氧化碳，5%。温度：37摄氏度、�/span>
供应限制 Permits and Restrictions	A。仅供研究之用、�/span>
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