| è´§å· | å“牌 | 产å“å称 | è§„æ ¼ | 包装ã€å‚è€ƒä»·æ ¼ | |
| L832365 | MACKLIN | 溶葡çƒèŒé…¶ | BR，1200u/mg | 521å…ƒ/1mg; 1888å…ƒ/5mg; 3400å…ƒ/10mg; 7999å…ƒ/25mg; 17100å…ƒ/100mg; | 咨询 |
储å˜ï¼š-20°C 状æ€ï¼šç™½è‰²åˆ°é»„色粉末 |
| L7386 | Sigma-Aldrich | Lysostaphin from Staphylococcus staphylolyticus | lyophilized powder, Protein 50-70 % by biuret, ≥500 units/mg protein | 1363.25å…ƒ/1 MG; 4176.42å…ƒ/5 MG; 10229.6å…ƒ/15 MG; | 咨询 |
产å“说明 一般æè¿° 化å¦ç»“构：多肽 包装 1, 5, 15 mg in glass bottle 生化/生ç†ä½œç”¨ 溶葡è„çƒèŒç´ 是一ç§åˆ†åé‡çº¦ä¸º25 kDa的锌内肽酶。 由于溶葡è„çƒèŒé…¶èƒ½è£‚解 è‘¡è„çƒèŒ 类细胞å£è‚½èšç³–层ä¸çš„èšç”˜æ°¨é…¸äº¤è”键，它已被å‘现å¯ç”¨äºŽç»†èƒžè£‚解，并具有潜在的抗èŒæ²»ç–—作用。
活性最佳pH：~7.5 å•ä½å®šä¹‰ 一个å•ä½åœ¨25℃和pH 7.5æ¡ä»¶ä¸‹ï¼Œåœ¨6.0 må应混åˆç‰©ä¸å¯ä½¿ 金黄色酿脓葡è„çƒèŒ 细胞悬浮液的浊度(A620)在10分钟内由0.250é™è‡³0.125。 åŸºæœ¬ä¿¡æ¯ | é…¶å¦å§”员会(EC)ç¼–å· | 3.4.24.75 ( BRENDA | IUBMB ) | | MDLç¼–å· | MFCD00131556 | | NACRES | NA.54 |
产å“性质 | 生物æ¥æº | microbial (Staphylococcus staphylotyticus) | | è´¨é‡æ°´å¹³ | 300 | | å½¢å¼ | lyophilized powder | | specific activity | ≥500 units/mg protein | | 分åé‡ | 25 kDa | | ç»„æˆ | Protein, 50-70% biuret | | 包装 | pkg of (sold on basis of mg protein) | | 溶解性 | water: 2.00-2.10 mg/mL, clear to slightly hazy, colorless to light yellow | | æŠ—ç”Ÿç´ æŠ—èŒè°± | Gram-positive bacteria | | application(s) | diagnostic assay manufacturing
diagnostic assay manufacturing | | 作用机制 | cell wall synthesis | interferes | | è¿è¾“ | wet ice | | 储å˜æ¸©åº¦ | −20℃ |
å®‰å…¨ä¿¡æ¯ | 象形图 |  | | è¦ç¤ºç”¨è¯ï¼š | Danger | | å±é™©å£°æ˜Ž | H334 | | 预防措施声明 | P261 - P284 - P304 + P340 + P312 - P501 | | å±é™©åˆ†ç±» | Resp. Sens. 1 | | 储å˜åˆ†ç±»ä»£ç | 11 - Combustible Solids | | WGK | WGK 3 | | 闪点(F) | Not applicable | | 闪点(C) | Not applicable | | 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
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| L2898 | Sigma-Aldrich | Lysostaphin from Staphylococcus staphylolyticus | aseptically filled | 4369.5元/1 MG; | 咨询 |
产å“说明 一般æè¿° 溶葡è„çƒèŒé…¶ä¸»è¦ä»¥é…¶åŽŸçš„å½¢å¼é‡Šæ”¾å‡ºæ¥ã€‚在 pH 4 和温度 5℃ 时高度稳定。
化å¦ç»“构：肽 生化/生ç†ä½œç”¨ 溶葡è„çƒèŒé…¶è¡¨çŽ°å‡ºå¯¹ 金黄色葡è„çƒèŒçš„溶解作用 。它拥有三ç§ä¸»è¦é…¶ç”˜æ°¨é…¸å†…肽酶(溶葡è„çƒèŒé…¶ã€å†…切β- N -乙酰基葡è„糖苷酶和 N -乙酸乙酯基- L -丙氨酸酰胺酶)çš„æ•°ç§åŠŸèƒ½ã€‚溶葡è„çƒèŒé…¶å¯ç”¨äºŽæ²»ç–—è€æŠ—ç”Ÿç´ çš„è‘¡è„çƒèŒæ„ŸæŸ“。溶葡è„çƒèŒé…¶æ˜¯ä¸€ç§æŠ—è‘¡è„çƒèŒå‰‚，å¯ç”¨ä½œé£Ÿå“工业和临床实验室的防è…剂，用于快速ç›æŸ¥ã€‚
溶葡è„çƒèŒç´ 是一ç§åˆ†åé‡çº¦ä¸º25 kDa的锌内肽酶。 由于溶葡è„çƒèŒé…¶èƒ½è£‚解 è‘¡è„çƒèŒ 类细胞å£è‚½èšç³–层ä¸çš„èšç”˜æ°¨é…¸äº¤è”键，它已被å‘现å¯ç”¨äºŽç»†èƒžè£‚解，并具有潜在的抗èŒæ²»ç–—作用。
活性最佳pH：~7.5 å•ä½å®šä¹‰ 在 pH 7.5ã€37℃ã€6.0 mL å应混åˆæ¶²ä¸ï¼Œ1 个å•ä½å¯åœ¨ 10 min 内将 金黄色葡è„çƒèŒ 细胞混悬液的浊度 (A 620 ) 从 0.250 é™è‡³ 0.125。 制备说明 从L 7386制备 åŸºæœ¬ä¿¡æ¯ | é…¶å¦å§”员会(EC)ç¼–å· | 3.4.24.75 ( BRENDA | IUBMB ) | | MDLç¼–å· | MFCD00131556 |
产å“性质 | æ— èŒæ€§ | aseptically filled | | è´¨é‡æ°´å¹³ | 200 | | å½¢å¼ | powder | | specific activity | >500 units/mg protein | | 分åé‡ | 25 kDa | | ç»„æˆ | Protein, 40-70% biuret | | 作用机制 | cell wall synthesis | interferes | | æŠ—ç”Ÿç´ æŠ—èŒè°± | Gram-positive bacteria | | 储å˜æ¸©åº¦ | −20℃ |
å®‰å…¨ä¿¡æ¯ | 象形图 | | | è¦ç¤ºç”¨è¯ï¼š | Danger | | å±é™©å£°æ˜Ž | H334 | | 预防措施声明 | P261 - P284 - P501 | | å±é™©åˆ†ç±» | Resp. Sens. 1 | | 储å˜åˆ†ç±»ä»£ç | 11 - Combustible Solids | | WGK | WGK 3 | | 闪点(F) | Not applicable | | 闪点(C) | Not applicable | | 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
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| L9043 | Sigma-Aldrich | Lysostaphin from Staphylococcus simulans | recombinant, expressed in E. coli, lyophilized powder | 4176.42元/5 MG; | 咨询 |
产å“说明 一般æè¿° 溶葡è„çƒèŒé…¶æ˜¯ä¸€ç§é”Œé‡‘属酶，主è¦ä»¥é…¶åŽŸçš„å½¢å¼åˆ†æ³Œå‡ºæ¥ã€‚它å¯ç”¨ä½œæŠ—金黄色葡è„çƒèŒå‰‚，在pH4å’Œ5℃时高度稳定。 应用 æ¥è‡ªæ‹Ÿè‘¡è„çƒèŒçš„溶葡çƒèŒé…¶è¢«ç”¨äºŽè¯„ä¼°lytM(一ç§ç‹¬ç‰¹çš„金黄色葡è„çƒèŒè‡ªæº¶åŸºå› )的分å克隆ã€æµ‹åºå’Œè¡¨è¾¾ã€‚ å®ƒè¿˜è¢«ç”¨äºŽç ”ç©¶é‡‘é»„è‰²è‘¡è„çƒèŒä¸ç¼–ç 肽èšç³–æ°´è§£é…¶æ´»æ€§çš„åŸºå› çš„åºåˆ—分æžã€‚ 包装 5 mg in glass bottle 生化/生ç†ä½œç”¨ 通过与金黄色葡è„çƒèŒçš„膜相关Trap蛋白的C′ 端结åˆï¼Œæº¶è‘¡çƒèŒé…¶(金黄色葡è„çƒèŒè¡¨è¾¾çš„一ç§é‡‘属内肽酶)的抗微生物活性得到增强。
一ç§è›‹ç™½å¤åˆä½“，对葡è„çƒèŒå±ž(包括金黄色葡è„çƒèŒ)具有高度特异性裂解活性。
溶葡è„çƒèŒé…¶å…·æœ‰æŠ—金黄色葡è„çƒèŒçš„裂解作用，å¯ç”¨äºŽæ²»ç–—è€æŠ—ç”Ÿç´ çš„è‘¡è„çƒèŒæ„ŸæŸ“。它具有甘氨酰甘氨酸内肽酶ã€å†…-β-N-乙酰葡è„糖胺酶和N-乙酰胞å£é…°-L-丙氨酸酰胺酶ç‰å¤šç§é…¶çš„特性。溶葡è„çƒèŒé…¶è¿˜å¯ç”¨äºŽé£Ÿå“防è…和临床实验室的快速ç›æŸ¥ã€‚ åŸºæœ¬ä¿¡æ¯ | é…¶å¦å§”员会(EC)ç¼–å· | 3.4.24.75 ( BRENDA | IUBMB ) | | MDLç¼–å· | MFCD00131556 | | NACRES | NA.54 |
产å“性质 | è´¨é‡æ°´å¹³ | 200 | | é‡ç»„ | expressed in E. coli | | 测定 | ≥90% (HPLC) | | å½¢å¼ | lyophilized powder | | specific activity | ≥3000 unit/mg solid | | è¿è¾“ | dry ice | | 储å˜æ¸©åº¦ | −20℃ |
å®‰å…¨ä¿¡æ¯ | 象形图 | | | è¦ç¤ºç”¨è¯ï¼š | Danger | | å±é™©å£°æ˜Ž | H334 | | 预防措施声明 | P261 - P284 - P501 | | å±é™©åˆ†ç±» | Resp. Sens. 1 | | 储å˜åˆ†ç±»ä»£ç | 11 - Combustible Solids | | WGK | WGK 3 | | 闪点(F) | Not applicable | | 闪点(C) | Not applicable | | 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
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| L4402 | Sigma-Aldrich | Lysostaphin from Staphylococcus staphylolyticus | BioUltra, ≥97% (SDS-PAGE), Protein 40-60 % by biuret, ≥2,000 units/mg protein | 2509.8å…ƒ/0.5 MG; 6860.56å…ƒ/2 MG; 15000.8å…ƒ/5 MG; | 咨询 |
产å“说明 一般æè¿° 包装尺寸基于蛋白质å«é‡
化å¦ç»“构：肽 包装 0.5, 2, 5 mg in glass bottle 生化/生ç†ä½œç”¨ 溶葡è„çƒèŒç´ 是一ç§åˆ†åé‡çº¦ä¸º25 kDa的锌内肽酶。 由于溶葡è„çƒèŒé…¶èƒ½è£‚解 è‘¡è„çƒèŒ 类细胞å£è‚½èšç³–层ä¸çš„èšç”˜æ°¨é…¸äº¤è”键，它已被å‘现å¯ç”¨äºŽç»†èƒžè£‚解，并具有潜在的抗èŒæ²»ç–—作用。
活性最佳pH：~7.5 å•ä½å®šä¹‰ 一个å•ä½åœ¨ 25℃ å’Œ pH 7.5 æ¡ä»¶ä¸‹ï¼Œåœ¨ 6.0 ml å应混åˆç‰©ä¸å¯ä½¿é‡‘黄色酿脓葡è„çƒèŒç»†èƒžæ‚¬æµ®æ¶²çš„浊度(A620)在 10 分钟内由 0.250 é™è‡³ 0.125。 外形 å«æœ‰ç£·é…¸é’¾ç¼“冲ç›å’Œæ°¯åŒ–é’ åˆ¶å¤‡è¯´æ˜Ž 亲和纯化 åŸºæœ¬ä¿¡æ¯ | é…¶å¦å§”员会(EC)ç¼–å· | 3.4.24.75 ( BRENDA | IUBMB ) | | MDLç¼–å· | MFCD00131556 | | NACRES | NA.54 |
产å“性质 | 产å“线 | BioUltra | | è´¨é‡æ°´å¹³ | 300 | | 测定 | ≥97% (SDS-PAGE) | | å½¢å¼ | lyophilized powder | | specific activity | ≥2,000 units/mg protein | | 分åé‡ | 25 kDa | | ç»„æˆ | Protein, 40-60% biuret | | 作用机制 | cell wall synthesis | interferes | | æŠ—ç”Ÿç´ æŠ—èŒè°± | Gram-positive bacteria | | 储å˜æ¸©åº¦ | −20℃ |
å®‰å…¨ä¿¡æ¯ | 象形图 | | | è¦ç¤ºç”¨è¯ï¼š | Danger | | å±é™©å£°æ˜Ž | H334 | | 预防措施声明 | P261 - P284 - P304 + P340 + P312 - P501 | | å±é™©åˆ†ç±» | Resp. Sens. 1 | | 储å˜åˆ†ç±»ä»£ç | 11 - Combustible Solids | | WGK | WGK 3 | | 闪点(F) | Not applicable | | 闪点(C) | Not applicable | | 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
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| SRE0053 | Sigma-Aldrich | Lysostaphin from Staphylococcus staphylolyticus | Suitable for manufacturing of diagnostic kits and reagents., lyophilized powder | 4896.14元/5 MG; | 咨询 |
产å“说明 一般æè¿° Lysostaphin is a glycylglycine endopeptidase. It is an important antibacterial enzyme. 生化/生ç†ä½œç”¨ Lysostaphin has the ability to cleave both viable and heat-killed S. aureus cells. It is one of the major antibacterial agents for the staphylococci. Lysostaphin cleaves the cross-linking pentaglycine bridges in the cell walls of staphylococci. Thus it is considered as an antistaphylococcal therapeutic agent.
溶葡è„çƒèŒç´ 是一ç§åˆ†åé‡çº¦ä¸º25 kDa的锌内肽酶。 由于溶葡è„çƒèŒé…¶èƒ½è£‚解 è‘¡è„çƒèŒ 类细胞å£è‚½èšç³–层ä¸çš„èšç”˜æ°¨é…¸äº¤è”键，它已被å‘现å¯ç”¨äºŽç»†èƒžè£‚解，并具有潜在的抗èŒæ²»ç–—作用。
活性最佳pH：~7.5 å•ä½å®šä¹‰ One unit will reduce the turbidity (A620) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH7.5 at 37℃ in a 6.0ml reaction mixture. åŸºæœ¬ä¿¡æ¯ | é…¶å¦å§”员会(EC)ç¼–å· | 3.4.24.75 ( BRENDA | IUBMB ) | | MDLç¼–å· | MFCD00131556 | | NACRES | NA.32 |
产å“性质 | è´¨é‡æ°´å¹³ | 400 | | å½¢å¼ | lyophilized powder | | specific activity | ≥500 units/mg protein | | 分åé‡ | 26,926 Da | | ç»„æˆ | protein, 50-70% biuret | | 包装 | pkg of (package size sold on basis of mg protein) | | è¿è¾“ | wet ice | | 储å˜æ¸©åº¦ | −20℃ |
å®‰å…¨ä¿¡æ¯ | 象形图 | | | è¦ç¤ºç”¨è¯ï¼š | Danger | | å±é™©å£°æ˜Ž | H334 | | 预防措施声明 | P261 - P284 - P501 | | å±é™©åˆ†ç±» | Resp. Sens. 1 | | 储å˜åˆ†ç±»ä»£ç | 11 - Combustible Solids | | WGK | WGK 3 | | 闪点(F) | Not applicable | | 闪点(C) | Not applicable |
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| SAE0091 | Sigma-Aldrich | Lysostaphin from Staphylococcus staphylolyticus | free of DNA contaminants, suitable for Microbiome research, lyophilized powder, ≥500 units/mg protein | 2759å…ƒ/2 MG; | 咨询 |
产å“说明 应用 Purified extracellular lysostaphin from S. staphylolyticus SAE0091 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Lysostaphin is a zinc metalloenzyme isolated from a bacterial culture of Staphylococcus staphylolyticus. It has specific lytic action against other Staphylococcus species, including S. aureus.1,2 Lysostaphin has
hexosaminidase, amidase, and endopeptidase activities. It cleaves polyglycine crosslinks in the cellular wall which leads to cell lysis of Staphylococcus species, but not of other bacterial genera. [Pharmaceuticals 2010, 3(4), 1139-1161]
Lysostaphin is a single polypeptide chain of 246 amino acids, a molecular mass of 26,926 Da, isoelectric point of 9.5, (5) and an activity pH optimum of 7.5.(6) 生化/生ç†ä½œç”¨ Lysostaphin is a zinc endopeptidase with a molecular weight of approximately 25 kDa. Because lysostaphin cleaves the polyglycine cross-links in the peptidoglycan layer of the cell wall of Staphylococcus species it has been found useful for cell lysis and also as a potential anti-microbial therapeutic. pH Optimum for activity: ~7.5.
溶葡è„çƒèŒç´ 是一ç§åˆ†åé‡çº¦ä¸º25 kDa的锌内肽酶。 由于溶葡è„çƒèŒé…¶èƒ½è£‚解 è‘¡è„çƒèŒ 类细胞å£è‚½èšç³–层ä¸çš„èšç”˜æ°¨é…¸äº¤è”键，它已被å‘现å¯ç”¨äºŽç»†èƒžè£‚解，并具有潜在的抗èŒæ²»ç–—作用。
活性最佳pH：~7.5 å•ä½å®šä¹‰ One unit will reduce the turbidity (A620) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37℃ in a 6.0 ml reaction mixture. 外形 Supplied as a lyophilized powder containing 50–70% protein with the balance primarily as NaCl. åŸºæœ¬ä¿¡æ¯ | é…¶å¦å§”员会(EC)ç¼–å· | 3.4.24.75 ( BRENDA | IUBMB ) | | MDLç¼–å· | MFCD00131556 |
产å“性质 | 生物æ¥æº | fermentation/recombinant | | å½¢å¼ | lyophilized powder | | specific activity | ≥500 units/mg protein | | 分åé‡ | 27 kDa | | 特点 | DNA free | | è¿è¾“ | wet ice | | 储å˜æ¸©åº¦ | −20℃ |
å®‰å…¨ä¿¡æ¯ | 象形图 | | | è¦ç¤ºç”¨è¯ï¼š | Danger | | å±é™©å£°æ˜Ž | H334 | | 预防措施声明 | P261 - P284 - P304 + P340 + P312 - P501 | | å±é™©åˆ†ç±» | Resp. Sens. 1 | | 储å˜åˆ†ç±»ä»£ç | 11 - Combustible Solids | | WGK | WGK 3 | | 闪点(F) | Not applicable | | 闪点(C) | Not applicable |
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