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CAS: 76-54-0
分子式: C20H10Cl2O5
分子量: 401.20
熔点: 280℃
中文名称: 
2',7'-二氯-3',6'-二羟基螺[异苯并呋喃-1(3H),9'-(9H)占吨]-3-酮
2',7'-二氯荧光素
2,7-二氯荧光黄
2,7-二氯-3,6-萤烷二醇
2,7-二氯荧光素
英文名称: 
2',7'-Dichlorofluorescein solution
2,7-Dichlorofluorescein
2',t-dichlorofluorescein
dc
dc orange 25(fluorescein
dc orange 25(fluorescein 548)
dcorange25(fluorescein548)
9'-[9H]xanthen]-3-one, 2',7'-dichloro-3',6'-dihydroxy-Spiro[isobenzofuran-1(3H)
2',7'-dichlorofluorescein
spiro[isobenzofuran-1(3h),9'-[9h]xanthen]-3-one, 2',7'-dichloro-3',6'-dihydroxy
Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one,2',7'-dichloro-3',6'-dihydroxy-
展开更多英文名
推荐供应商
货号品牌产品名称规格包装、参考价格
D807623MACKLIN2',7'-二氯荧光素指示剂, ≥90%56元/1g;   185元/5g;   698元/25g;   2496元/100g;   咨询
储存:室温
状态:橙色到红棕色粉末
D828330MACKLIN2',7'-二氯荧光素65%45元/1g;   145元/5g;   502元/25g;   1800元/100g;   咨询
储存:室温
状态:橙色至红色棕色粉末
D909945MACKLIN2',7'-二氯荧光素98%152元/1g;   412元/5g;   1218元/25g;   2880元/100g;   8790元/500g;   咨询
储存:室温
状态:浅黄色到棕色粉末到晶体
D0371TCI2',7'-Dichlorofluorescein [for Fluorescent indicator]>95.0%(HPLC)175元/1G;   1485元/25G;   咨询

基本信息

纯度/分析方法>95.0%(HPLC)
分子式/分子量C20H10Cl2O5 = 401.20
外观与形状(20℃)固体
Reaxys-RN58009
PubChem物质ID87567058
SDBS (AIST Spectral DB)5116
MDL编号MFCD00005047

技术规格

AppearanceLight yellow to Brown powder to crystal
Purity(HPLC)min. 95.0 area%
Drying lossmax. 10.0 %
Sensitivenessto pass test

物性(参考值)

熔点280 ℃(dec.)
溶解性(可溶于)氢氧化钠水溶液
溶解性(微溶于)乙醇

安全信息

图形或危害标志
信号词警告
危险性说明H315 : 造成皮肤刺激。
H319 : 造成严重眼刺激。
防范说明P264 : 作业后彻底清洗皮肤。
P280 : 戴防护手套/戴防护眼罩/戴防护面具。
P302 + P352 : 如皮肤沾染:用水充分清洗。
P337 + P313 : 如仍觉眼刺激:求医/就诊。
P305 + P351 + P338 : 如进入眼睛:用水小心冲洗几分钟。如戴隐形眼镜并可方便地取出,取出隐形眼镜。继续冲洗。
P362+P364 : 脱掉沾污的衣服,清洗后方可重新使用。
P332 + P313 : 如发生皮肤刺激:求医/就诊。
223219J&K2',7'-Dichlorofluorescein152元/1G;   317元/5G;   1264元/25G;   咨询

基本信息

分子式C20H10Cl2O5
分子量401.20
熔点280
密度0.79
MDL编码MFCD00005047
Beilstein58009
EINECS 编号200-968-6

荧光性质

λex 504 nm; λem 529 nm in 0.1 M Tris pH 8.0

应用

2′,7′-二氯荧光素二乙酸酯可用作未衍生化荧光素的优良气相替代物,并用作 ′,7′-二氯荧光素二乙酸酯测定的组分/底物,定量活性氧物质(ROS)。

安全信息

图形或危害标志
提示语Warning
危险说明H315
H319
H335
防范说明P261
P305+P351+P338
WGK3
TSCA
A16212Alfa Aesar2',7'-Dichlorofluorescein696元/5g;   2277元/25g;   咨询

应用
2',7'-Dichlorofluorescein is used as an indicator and as a cellular antioxidant activity. It is also used for the determination of carbohydrates and hydrogen peroxide by fluorescence densitometry and chemiluminescence respectively. It is used as an indicator for argentometry by Fajans method. It is also involved in the cellular antioxidant activity assay. It is utilized as an additive in fluorescence and laser studies.

备注
As a fluorescent indicatorIncompatible with strong oxidizing agents.

基本信息

MDL
MFCD00005047

EINECS
200-968-6

分子式
C20H10Cl2O5

分子量
401.20

熔点
>300° dec.

灵敏度
Ambient temperatures.

形态
Powder

溶解性
Soluble in ethanol and methanol. Slightly dimethyl sulfoxide. Insoluble in water.

GHS危害和防范说明

GHS符号

Hazard Statements
H319
Causes serious eye irritation.

Precautionary Statements
P280i-P264-P305+P351+P338-P337+P313
Wear eye/face protection.Wash thoroughly after handling.IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.If eye irritation persists: Get medical advice/attention.

安全信息

危险类别
36
Irritating to eyes.

安全等级
26
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.

TSCA

C19153Alfa Aesar2',7'-Dichlorofluoresceinpure1569元/5g;   5249元/25g;   15525元/100g;   咨询

基本信息

Ref#
19153

MDL
5047

分子式
C20 H10 Cl2 O5

分子量
401.2

密度
0.79

形态
Orange powder

C44693Alfa Aesar2',7'-DichlorofluoresceinACS reagent752元/1g;   3550元/10g;   咨询

基本信息

Ref#
44693

MDL
5047

分子式
C20 H10 Cl2 O5

分子量
401.2

密度
0.79

形态
Orange powder

410217Sigma-Aldrich2′,7′-DichlorofluoresceinACS reagent531.78元/1 G;   2476.47元/10 G;   咨询

基本信息

经验(实验)分子式C20H10Cl2O5
分子量401.20
Beilstein58009
EC 号200-968-6
MDL编号MFCD00005047
PubChem化学物质编号24865700
NACRESNA.47

产品性质

质量水平200
等级ACS reagent
形式powder
颜色 orange to red-brown, powder
mp280 ℃ (dec.) (lit.)
溶解性alcohol: passes test
密度0.790 g/cm3
λmax509 nm
适用性passes test for adsorption indicator
application(s)diagnostic assay manufacturing
hematology
histology
储存温度room temp
SMILES stringOc1cc2Oc3cc(O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl
InChI1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H
InChI keyVFNKZQNIXUFLBC-UHFFFAOYSA-N

安全信息

储存分类代码13 - Non Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备dust mask type N95 (US), Eyeshields, Gloves
1.09676Supelco2′,7′-Dichlorofluoresceinindicator ACS,Reag. Ph Eur3231.81元/5 G;   咨询

产品说明

分析说明

Clarity of solution (according to ACS): passes test
Loss on drying (110 °C): ≤ 3
Suitability as adsorption indicator (according to ACS): passes test

基本信息

经验(实验)分子式C20H10Cl2O5
分子量401.20
MDL编号MFCD00005047
EC索引号200-968-6

产品性质

质量水平200
等级ACS reagent
agencyreag. Ph. Eur.
缺失≤3% loss on drying, 110℃
堆积密度390 kg/m3
储存温度2-30℃
InChI1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H
InChI keyVFNKZQNIXUFLBC-UHFFFAOYSA-N

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
D6665Sigma-Aldrich2′,7′-Dichlorofluorescein~90% (TLC), powder997.51元/5 g;   4675.14元/25 g;   咨询

产品说明

应用

2 & # 8242;,7 & # 8242;-二氯荧光素已被用作氧化敏感荧光探针来测量细胞中活性氧(活性氧)的形成。

生化/生理作用

2′,7′-二氯荧光素是一种氧化敏感荧光探针。2′7′-二氯荧光素二乙酸酯被氧化成2′7′-在活性氧(活性氧)存在下的二氯荧光素。

基本信息

经验(实验)分子式C20H10Cl2O5
分子量401.20
Beilstein58009
EC 号200-968-6
MDL编号MFCD00005047
PubChem化学物质编号24894041
NACRESNA.47

产品性质

测定~90% (TLC)
质量水平200
形式crystalline
mp280 ℃ (dec.) (lit.)
溶解性ethanol: 25 mg/mL
密度0.79 g/cm3
application(s)diagnostic assay manufacturing
hematology
histology
储存温度room temp
SMILES stringOc1cc2Oc3cc(O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl
InChI1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H
InChI keyVFNKZQNIXUFLBC-UHFFFAOYSA-N

安全信息

储存分类代码13 - Non Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备dust mask type N95 (US), Eyeshields, Gloves
35848Sigma-Aldrich2′,7′-DichlorofluoresceinBioReagent, suitable for fluorescence, ≥90% (T)1054.64元/5 G;   5392.09元/25 G;   咨询

产品说明

应用

要制备 2′,7′-二氯荧光素二乙酸酯:

溶液 A:溶解在冰醋酸中的饱和四乙酸铅溶液(约 2%,货号 15370)
溶液 B:2′,7′-二氯荧光素溶于乙醇 (0.2-1%)
在使用之前,将 5 mL 溶液 A 与 5 mL 溶液 B 混合,并添加甲苯至 200 mL。溶液应现配。浸渍时间:10 秒。

2′,7′-二氯荧光素二乙酸酯可用作未衍生化荧光素的优良气相替代物,并可用作 2′,7′-二氯荧光素二乙酸酯测定的组分/底物,以定量活性氧物质 (ROS)。也可作为预浸剂,用于荧光测定邻位二醇、糖苷和酚的预浸剂。

基本信息

经验(实验)分子式C20H10Cl2O5
分子量401.20
Beilstein58009
EC 号200-968-6
MDL编号MFCD00005047
PubChem化学物质编号57649439
NACRESNA.32

产品性质

质量水平100
产品线BioReagent
测定≥90% (T)
mp280 ℃ (dec.) (lit.)
荧光λex 504 nm; λem 529 nm in 0.1 M Tris pH 8.0
适用性suitable for fluorescence
SMILES stringOc1cc2Oc3cc(O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl
InChI1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H
InChI keyVFNKZQNIXUFLBC-UHFFFAOYSA-N

安全信息

储存分类代码13 - Non Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备dust mask type N95 (US), Eyeshields, Gloves
955911Amethyst2',7'-Dichlorofluorescein98%, 试剂级503元/5G;   1395元/25G;   4597元/100G;   咨询

基本信息

分子式C20H10Cl2O5
分子量401.20
熔点280
密度0.79
MDL编码MFCD00005047
Beilstein58009
EINECS 编号200-968-6

安全信息

图形或危害标志
提示语Warning
危险说明H315
H319
H335
防范说明P261
P305+P351+P338
WGK3
TSCA
M05763Fluorochem2',7'-Dichlorofluorescein>95.0%(HPLC)528元/1g;   3190元/25g;   咨询

基本信息

CAS Number76-54-0
MDL NumberMFCD00005047
Melting Point280℃

安全信息

图形或危害标志
危险性说明 H302: Harmful if swallowed.
H315: Causes skin irritation.
H319: Causes serious eye irritation.
H335: May cause respiratory irritation.
防范说明 P233: Keep container tightly closed.
P260: Do not breathe .
P261: Avoid breathing
P264: Wash hands thoroughly after handling.
P270: Do not eat, drink or smoke when using this product.
P271: Use only outdoors or in a well-ventilated area.
P280: Wear .
P301 + P312: IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell.
P302 + P352: IF ON SKIN: Wash with plenty of soap and water.
P304: IF INHALED: .
P304 + P340: IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing.
P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P312: Call a POISON CENTER or doctor/physician if you feel unwell.
P321: Specific treatment (see . on this label).
P330: Rinse mouth.
P332 + P313: If skin irritation occurs: Get medical advice/ attention.
P337 + P313: If eye irritation persists: Get medical advice/attention.
P340: Remove victim to fresh air and keep at rest in a position comfortable for breathing.
P362: Take off contaminated clothing and wash before reuse.
P403: Store in a well-ventilated place.
P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
P405: Store locked up.
P501: Dispose of contents/container to local regulations.
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