产品说明

一般描述

A cell-permeable furoic acid compound that acts as a potent, reversible, and competitive inhibitor of acetyl-CoA carboxylase (ACC), a key enzyme involved in the fatty acid biosynthesis. Inhibits cellular fatty acid synthesis in a dose-dependent manner (IC₅₀ = 4 µM in human breast cancer cell line MCF7). TOFA-induced reduction in malonyl-CoA is reported to off-set the effect of C75 (Cat. No. 341325) on food intake in fasted mice and on apoptosis in tumor cells.
A cell-permeable, potent, reversible, and competitive inhibitor of acetyl-CoA carboxylase (ACC), a key enzyme involved in fatty acid biosynthesis. Inhibits cellular fatty acid synthesis in a dose-dependent manner (IC₅₀ = 4 µM in human breast cancer cell line MCF7). TOFA-induced reduction in malonyl-CoA is reported to off-set the effects of C75 (Cat. No. 341325) on food intake in fasted mice and on apoptosis in tumor cells.

包装

5 mg in Plastic ampoule
Packaged under inert gas

生化/生理作用

Primary Target
Acetyl-CoA carboxylase (ACC)
Target IC₅₀: 4 µM inhibiting cellular fatty acid synthesis in human breast cancer cell line MCF7
Reversible: yes
Cell permeable: yes
Product competes with ATP.

警告

Toxicity: Carcinogenic / Teratogenic (D)

制备说明

Slight warming and sonication may be required for complete solubilization in ethanol.

重悬

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

其他说明

Landree, L.E., et al. 2004. J. Biol. Chem.279, 3817.
Zhou, W., et al. 2003. Cancer Res.63, 7330.
Hu, Z., et al. 2003. Proc. Natl. Acad. Sci. USA100, 12624.
Pizer, E.S., et al. 2000. Cancer Res.60, 213.
Arbeeny, C.M., et al. 1992. J. Lipid Res.33, 843.
Fukuda, N. and Ontko, J.A. 1984. J. Lipid Res.25, 831.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

产品性质

安全信息

产品说明

应用

TOFA用作可用作：间充质基质细胞(MSC)、人类多功能干细胞中的脂类生化合成抑制剂。小鼠脂肪细胞系中的乙酰-辅酶A羧化酶1抑制剂。一种癌症干细胞(CSC)的脂解抑制剂。
TOFA已被用作 ACC抑制剂，以研究其对 INS-1E细胞中胰岛素分泌的影响。

包装

5 mg in glass bottle
25 mg in poly bottle

生化/生理作用

研究报道，在大鼠再喂食实验期间，TOFA可以抑制皮下他莫昔芬(TMX)对食物摄入的厌食作用。
TOFA是乙酰辅酶A 羧化酶(ACC)的细胞渗透性、有效、可逆和竞争性抑制剂。 TOFA是参与脂肪酸生物合成的关键酶。TOFA以剂量依赖性方式抑制细胞脂肪酸合成(人乳腺癌细胞系 MCF7中IC₅₀ = 4 μM)。
5-(十四环氧基)-2-呋喃甲酸(TOFA)可促进脂肪酸分解并阻止生物合成，实现降血脂功能。它能诱导胰腺癌细胞凋亡，促进肿瘤抑制。

制备说明

TOFA以大于2mg/ml的浓度溶于DMSO。

基本信息

产品性质

安全信息