| 货号 | 品牌 | 产品名称 | 规格 | 包装、参考价格 | |
| I796880 | MACKLIN | 异丙基-β-D-硫代半乳糖苷 | 10mM in DMSO | 185元/1ml; | 咨询 |
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| I885106 | MACKLIN | IPTG溶液 | 200mg/ml | 178元/1.5ml; 439元/5ml; 1678元/25ml; 4500元/100ml; | 咨询 |
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| I741654 | MACKLIN | IPTG溶液 | 500mM in H2O | 215元/5ml; 750元/25ml; 2250元/100ml; | 咨询 |
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| I811719 | MACKLIN | 异丙基-β-D-硫代半乳糖苷 | >98.0%(GC) | 33元/1g; 71元/5g; 254元/25g; 921元/100g; 2764元/500g; | 咨询 |
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| I6148 | MACKLIN | 异丙基-β-D-硫代半乳糖苷 | 98%生物技术级 | 50元/1g; 136元/5g; 516元/25g; 1798元/100g; 5980元/500g; | 咨询 |
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| 266729 | J&K | Isopropyl β-D-thiogalactopyranoside | 99%, 用于生物化学 | 423元/5G; 1716元/25G; 5147元/100g; | 咨询 |
基本信息 | 分子式 | C9H18O5S | | 分子量 | 238.30 | | 熔点 | 113 | | 比旋光度([α]20D) | -34.5° to -28.5° (C=1, water) | | MDL编码 | MFCD00063273 | | Beilstein | 4631 | | Merck | 14, 5082 | | EINECS 编号 | 206-703-0 | | 存储条件 | Store at 2-8℃ |
安全信息 | 图形或危害标志 |   | | 提示语 | Warning | | 危险说明 | H315 H319 H335 H351 | | 防范说明 | P281 P305+P351+P338 P302+P340 P312 | | WGK | 3 | | TSCA | 是 |
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| B21149 | Alfa Aesar | Isopropyl-beta-D-thiogalactoside | dioxane-free, 99% | 1451元/1g; 7255元/5g; | 咨询 |
应用 Beta-galactosidase inducer.Isopropyl-β-D-thiogalactoside is used as a reagent in molecular biology. It is used as an effective inducer of protein expression where the gene is under the control of the lac operator. It is used with 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside to identify the non-recombinant plasmid in cloning experiments by blue-white screen methodology. 备注 Hygroscopic. Store in a cool place. Incompatible with strong oxidizing agents. 基本信息 MDL MFCD00063273 EINECS 206-703-0 分子式 C9H18O5S 分子量 238.30 熔点 109-115° 灵敏度 Store at -20°C. 形态 Powder 溶解性 Soluble in water. Beilstein 4631 安全信息 TSCA 是 |
| C30279 | Alfa Aesar | Isopropyl-beta-D-thiogalactopyranoside | 99%, for biochemistry, dioxane-free | 1086元/1g; 7455元/10g; 9384元/25g; | 咨询 |
基本信息 Ref# 30279 MDL 63273 分子式 C9 H18 O5 S 分子量 238.31 |
| 420291 | Millipore | IPTG, Animal-Free, High Purity-CAS 367-93-1-Calbiochem | Inducer of β-D-galactosidase. Used in the stimulation of β-galactosidase in cellular systems in which dioxane would disrupt normal cell function. Contains no animal-derived products. | 1392.67元/1 G; 3401.22元/5 G; | 咨询 |
产品说明 一般描述 Inducer of β-D-galactosidase. Used in the stimulation of β-galactosidase in cellular systems in which dioxane would disrupt normal cell function. Contains no animal-derived products. 包装 1 g in Glass bottle 5 g in Plastic ampoule 警告 Toxicity: Standard Handling (A) 重悬 Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. 其他说明 Lama, J., et al. 1992. Gene117, 185. Zhang, Y., and Moss, B. 1991. Natl. Acad. Sci. USA88, 1511. Rodriquez, J.F., and Smith, G.L. 1990. Nucleic Acids Res.18, 5347. Date, T., et al. 1988. Biochem. Biophys. Res. Commun.151, 598. Lee, S.G., et al. 1988. Biochemistry27, 2983. Cho, S., et al. 1985. Biochem. Biophys. Res. Commun.128, 1268. 法律信息 CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany 基本信息 | 经验(实验)分子式 | C9H18O5S | | 分子量 | 238.30 | | MDL编号 | MFCD00063273 |
产品性质 | 质量水平 | 100 | | 测定 | ≥98% (HPLC) | | 形式 | solid | | manufacturer/tradename | Calbiochem® | | 储存条件 | OK to freeze desiccated (hygroscopic) | | 杂质 | Dioxane, none detected | | 颜色 | white to off-white | | 溶解性 | water: 100 mg/mL methanol: soluble | | 运输 | ambient | | 储存温度 | 2-8℃ | | InChI | 1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1 | | InChI key | BPHPUYQFMNQIOC-NXRLNHOXSA-N |
安全信息 | 储存分类代码 | 11 - Combustible Solids | | WGK | WGK 3 | | 闪点(F) | Not applicable | | 闪点(C) | Not applicable |
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| I6758 | Sigma-Aldrich | IPTG | ≥99% (TLC), ≤0.1% Dioxane | 1111.45元/1 G; 5109.92元/5 G; 8335.81元/10 G; | 咨询 |
产品说明 包装 1, 5, 10 g in glass bottle 应用 IPTG 常用于需要诱导 β-半乳糖苷酶活性的克隆实验。它常与 X-Gal 或 Bluo-Gal 结合使用,用于重组细菌菌落的蓝白筛选,这些菌落可以诱导 lac 操纵子在大肠杆菌中的表达。IPTG 与 lacI 阻遏蛋白结合并改变其构象而发挥作用,防止 β-半乳糖苷酶编码基因 lacZ 的抑制。 其他说明 非代谢性半乳糖类似物。 重悬 将 IPTG 溶于水,然后经无菌过滤,配制成储存液 (0.1M)。指示板中 IPTG 最终浓度应为 0.2mM。 基本信息 | 经验(实验)分子式 | C9H18O5S | | 分子量 | 238.30 | | Beilstein | 4631 | | EC 号 | 206-703-0 | | MDL编号 | MFCD00063273 | | PubChem化学物质编号 | 24896093 | | NACRES | NA.31 |
产品性质 | 质量水平 | 300 | | 生物来源 | synthetic | | 描述 | non-ionic | | 测定 | ≥99% (TLC) | | 形式 | powder | | technique(s) | thin layer chromatography (TLC): suitable | | 杂质 | ≤0.1% Dioxane | | 储存温度 | 2-8℃ | | SMILES string | CC(C)S[C@@H]1OC(CO)[C@H](O)C(O)[C@H]1O | | InChI | 1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1 | | InChI key | BPHPUYQFMNQIOC-NXRLNHOXSA-N |
安全信息 | 储存分类代码 | 11 - Combustible Solids | | WGK | WGK 3 | | 闪点(F) | Not applicable | | 闪点(C) | Not applicable | | 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
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| 420290 | Millipore | IPTG, Hemidioxane Adduct-CAS 367-93-1-Calbiochem | A free-flowing, hygroscopic carbohydrate that induces expression of the lac operon of E. coli by allosterically interacting with the repressor molecule. | 2941.04元/5 G; | 咨询 |
产品说明 一般描述 A free-flowing, hygroscopic carbohydrate that induces expression of the lac operon of E. coli by allosterically interacting with the repressor molecule. Inducer of β-D-galactosidase. This preparation contains 15% dioxane. A free flowing carbohydrate that induces expression of the lac operon of E. coli by allosterically interacting with the repressor molecule. Inducer of β-D-galactosidase. 包装 5 g in Plastic ampoule 警告 Toxicity: Carcinogenic / Teratogenic (D) 重悬 Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. 其他说明 Mathur, A., et al. 2000. Cardiovasc. Res. 46, 126. Chanda, B., and Mathew, M.K. 1999. Biochim Biophys. Acta 1416, 92. Dedov, V.N., and Roufogalis, B.D. 1999. FEBS Lett. 456, 171. Polla, B.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 6458. White, R.J., et al. 1996. J. Neurosci. 16, 5688. Ankacrona, M., et al. 1995. Neuron 15, 961. Reers, M., et al. 1995. Methods Enzymol. 260, 406. Ravanello, M.P., and Kruby, D.E. 1994. J. Virol.68, 6401. Schmitz, M.L., and Baeurerle, P.A. 1994. Biotechniques17, 714. Cossarizza, A., et al. 1993. Biochem. Biophys. Res. Commun. 197, 40. Lama, J., et al. 1992. Gene117, 185. Zhang, Y., and Moss, B. 1991. Proc. Natl. Acad. Sci. USA88, 1511. Rodriquez, J.F., and Smith, G.L. 1990. Nucleic Acids Res.18, 5347. Date, T., et al. 1988. Biochem. Biophys. Res. Commun.151, 598. Lee, S.G., et al. 1988. Biochemistry27, 2983. Cho, S., et al. 1985. Biochem. Biophys. Res. Commun.128, 1268. 法律信息 CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany 基本信息 | 经验(实验)分子式 | C9H18O5S · 0.5C4H8O2 | | 分子量 | 282.35 | | MDL编号 | MFCD00063273 |
产品性质 | 质量水平 | 100 | | 测定 | ≥98% (HPLC) | | 形式 | solid | | manufacturer/tradename | Calbiochem® | | 储存条件 | OK to freeze desiccated (hygroscopic) | | 杂质 | 15.0% dioxane | | 颜色 | white to off-white | | 溶解性 | water: 1% ethanol: soluble | | 运输 | ambient | | 储存温度 | 2-8℃ | | InChI | 1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1 | | InChI key | BPHPUYQFMNQIOC-NXRLNHOXSA-N |
安全信息 | 储存分类代码 | 11 - Combustible Solids | | WGK | WGK 3 | | 闪点(F) | Not applicable | | 闪点(C) | Not applicable |
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| 420322 | Millipore | IPTG, Dioxane-Free, High Purity-CAS 367-93-1-Calbiochem | CAS 367-93-1 is used in the stimulation of β-galactosidase in cellular systems in which dioxane would disrupt normal cell function. | 1190.68元/1 G; 2997.23元/5 G; 4227.32元/10 G; 8858.6元/25 G; 21578.2元/100 G; 38980.6元/500 G; | 咨询 |
产品说明 一般描述 在二恶烷会破坏正常细胞功能的细胞系统中用于刺激 β-半乳糖苷酶。通过 GC 检测,该制剂包含 ≤0.1% 的二恶烷。 β-D-半乳糖苷酶的诱导剂。在二恶烷会破坏正常细胞功能的细胞系统中用于刺激 β-半乳糖苷酶。IPTG 诱导与 lac 启动子连接的克隆基因的表达。 另请参见 IPTG,无动物成分,高纯度,货号420291)中的25 mM溶液。 包装 5 g in Glass bottle 1 g in Plastic ampoule 100, 500 g in Glass bottle 10, 25 g in Plastic ampoule 警告 毒性:标准处理(A) 法律信息 CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany 基本信息 | 经验(实验)分子式 | C9H18O5S | | 分子量 | 238.30 | | MDL编号 | MFCD00063273 |
产品性质 | 质量水平 | 100 | | 测定 | ≥98% (HPLC) | | 形式 | solid | | manufacturer/tradename | Calbiochem® | | 储存条件 | OK to freeze desiccated (hygroscopic) protect from light | | 杂质 | ≤0.1% Dioxane (GC) | | 颜色 | white | | 溶解性 | water: 50 mg/mL | | 运输 | ambient | | 储存温度 | 2-8℃ | | InChI | 1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1 | | InChI key | BPHPUYQFMNQIOC-NXRLNHOXSA-N |
安全信息 | 储存分类代码 | 11 - Combustible Solids | | WGK | WGK 3 | | 闪点(F) | Not applicable | | 闪点(C) | Not applicable |
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| I5502 | Sigma-Aldrich | Isopropyl β-D-1-thiogalactopyranoside | ≥99% (TLC) | 581.52元/1 G; 1832.55元/5 G; 3488.86元/10 G; | 咨询 |
产品说明 包装 1, 5, 10 g in poly bottle 应用 IPTG 常用于需要诱导 β-半乳糖苷酶活性的克隆实验。它常与 X-Gal 或 Bluo-Gal 结合使用,用于重组细菌菌落的蓝白筛选,这些菌落可以诱导 lac 操纵子在大肠杆菌中的表达。IPTG 与 lacI 阻遏蛋白结合并改变其构象而发挥作用,防止 β-半乳糖苷酶编码基因 lacZ 的抑制。 其他说明 非代谢性半乳糖类似物。 重悬 将 IPTG 溶于水,然后经无菌过滤,配制成储存液 (0.1M)。指示板中 IPTG 最终浓度应为 0.2mM。 基本信息 | 经验(实验)分子式 | C9H18O5S | | 分子量 | 238.30 | | Beilstein | 4631 | | EC 号 | 206-703-0 | | MDL编号 | MFCD00063273 | | PubChem化学物质编号 | 24896069 | | NACRES | NA.31 |
产品性质 | 描述 | non-ionic | | 测定 | ≥99% (TLC) | | 形式 | powder | | technique(s) | thin layer chromatography (TLC): suitable | | 杂质 | 10.0-22.0% Dioxane | | 储存温度 | 2-8℃ | | SMILES string | CC(C)S[C@@H]1OC(CO)[C@H](O)C(O)[C@H]1O | | InChI | 1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1 | | InChI key | BPHPUYQFMNQIOC-NXRLNHOXSA-N |
安全信息 | 象形图 |   | | 警示用语: | Warning | | 危险声明 | H319 - H351 | | 预防措施声明 | P281 - P305 + P351 + P338 | | 危险分类 | Carc. 2 - Eye Irrit. 2 | | 储存分类代码 | 11 - Combustible Solids | | WGK | WGK 3 | | 闪点(F) | Not applicable | | 闪点(C) | Not applicable |
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| 70527 | Sigma-Aldrich | 100 mM IPTG Solution-Novagen | Convenient, ready-to-use solution | 2069.99元/15 ML; | 咨询 |
产品说明 一般描述 The lac operon inducer IPTG (isopropyl-β-D-1-thiogalactopyranoside) is recommendedfor blue/white screening by lacZ α-complementation with appropriate vectorsand host strains, such as pSTBlue-1/NovaBlue,and for inducing protein expression with thepET System and other lac promoter-derivedexpression systems. The preparation isdioxane-free and supplied as a sterile aqueoussolution, in convenient volumes (10 × 1.5 ml). 包装 15 ml in Glass bottle 警告 Toxicity: Carcinogenic / Teratogenic (D) 法律信息 NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany 基本信息 产品性质 | 质量水平 | 100 | | manufacturer/tradename | Novagen® | | 储存条件 | OK to freeze avoid repeated freeze/thaw cycles | | 运输 | wet ice | | 储存温度 | −20℃ | | InChI | 1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1 | | InChI key | BPHPUYQFMNQIOC-NXRLNHOXSA-N |
安全信息 | 储存分类代码 | 11 - Combustible Solids | | WGK | WGK 3 | | 闪点(F) | Not applicable | | 闪点(C) | Not applicable |
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| V900917 | | Isopropyl β-D-thiogalactoside | Vetec™, reagent grade, ≥99% | 191.89元/5 G; | 咨询 |
产品说明 包装 5 g in poly bottle 应用 IPTG 常用于需要诱导 β-半乳糖苷酶活性的克隆实验。它常与 X-Gal 或 Bluo-Gal 结合使用,用于重组细菌菌落的蓝白筛选,这些菌落可以诱导 lac 操纵子在大肠杆菌中的表达。IPTG 与 lacI 阻遏蛋白结合并改变其构象而发挥作用,防止 β-半乳糖苷酶编码基因 lacZ 的抑制。 其他说明 非代谢性半乳糖类似物。 法律信息 Vetec is a trademark of sigma-aldrich International GmbH 基本信息 | 经验(实验)分子式 | C9H18O5S | | 分子量 | 238.30 | | Beilstein | 4631 | | MDL编号 | MFCD00063273 | | PubChem化学物质编号 | 329830223 |
产品性质 | 等级 | reagent grade | | 产品线 | Vetec™ | | 测定 | ≥99% | | 储存温度 | 2-8℃ | | SMILES string | CC(C)S[C@@H]1OC(CO)[C@H](O)C(O)[C@H]1O | | InChI | 1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1 | | InChI key | BPHPUYQFMNQIOC-NXRLNHOXSA-N |
安全信息 | 储存分类代码 | 11 - Combustible Solids | | WGK | WGK 3 | | 闪点(F) | Not applicable | | 闪点(C) | Not applicable |
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| PHG0010 | SAFC | Isopropyl β-D-1-thiogalactopyranoside | | 5914.42元/5 G; 19572.7元/25 G; 65764.9元/100 G; 328810元/1000 G; | 咨询 |
产品说明 应用 IPTG 常用于需要诱导 β-半乳糖苷酶活性的克隆实验。它常与 X-Gal 或 Bluo-Gal 结合使用,用于重组细菌菌落的蓝白筛选,这些菌落可以诱导 lac 操纵子在大肠杆菌中的表达。IPTG 与 lacI 阻遏蛋白结合并改变其构象而发挥作用,防止 β-半乳糖苷酶编码基因 lacZ 的抑制。 其他说明 非代谢性半乳糖类似物。 基本信息 | 经验(实验)分子式 | C9H18O5S | | 分子量 | 238.30 | | Beilstein | 4631 | | EC 号 | 206-703-0 | | MDL编号 | MFCD00063273 | | PubChem化学物质编号 | 329823027 |
产品性质 | 质量水平 | 300 | | 生物来源 | synthetic | | 形式 | powder | | 储存温度 | 2-8℃ | | SMILES string | CC(C)S[C@@H]1OC(CO)[C@H](O)C(O)[C@H]1O | | InChI | 1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1 | | InChI key | BPHPUYQFMNQIOC-NXRLNHOXSA-N |
安全信息 | 储存分类代码 | 11 - Combustible Solids | | WGK | WGK 3 | | 闪点(F) | Not applicable | | 闪点(C) | Not applicable |
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| I1284 | Sigma-Aldrich | Isopropyl β-D-thiogalactopyranoside solution | ReadyMade IPTG solution for Blue-white screening | 1098.03元/5 ML; | 咨询 |
产品说明 一般描述 Isopropyl thiogalactose (IPTG) is a sugar derivative widely used for the induction of the expression of recombinant proteins in E. coli. This expression system requires that the gene of interest is cloned in plasmids containing the Lac promoter. IPTG Ready Made is a 0.2 μm filter sterilized solution of 200 mM IPTG. This reagent remains liquid at its storage temperature (-20 ℃), thus avoiding freeze-thaw cycles and providing a user-friendly solution. 应用 Isopropyl β-D-thiogalactopyranoside solution is:
- used as an inducer for the expression of single domain antibodies(sdAbs) in E coli and bioactive peptides
- used in its half-life and decay studies
- suitable for induction of recombinant protein expression in systems controlled by the lac repressor
生化/生理作用 Isopropyl β-D-thiogalactopyranoside binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner. This binding allows the transcription of genes in the lac operon, such as the gene coding for β--galactosidase. The β-galactosidase gene is transcribed and monosaccharides are the product. Since Isopropyl β-D-thiogalactopyranoside cannot be metabolized by E. coli its concentration remains constant. Therefore, it is not a variable in the experiment. 组分 IPTG Solution is composed of 200 mM IPTG in molecular biology grade water. 原理 Isopropyl β-D-thiogalactopyranoside binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner. This binding allows the transcription of genes in the lac operon, such as the gene coding for β-galactosidase. The β-galactosidase gene is transcribed and monosaccharides are the product. 基本信息 | 经验(实验)分子式 | C9H18O5S | | 分子量 | 238.30 | | Beilstein | 4631 | | MDL编号 | MFCD00063273 | | PubChem化学物质编号 | 24895928 | | NACRES | NA.52 |
产品性质 | 质量水平 | 200 | | 等级 | for molecular biology | | 描述 | non-ionic | | 形式 | liquid | | 运输 | dry ice | | 储存温度 | −20℃ | | SMILES string | CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O | | InChI | 1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1 | | InChI key | BPHPUYQFMNQIOC-NXRLNHOXSA-N |
安全信息 | 储存分类代码 | 12 - Non Combustible Liquids | | WGK | WGK 2 | | 闪点(F) | Not applicable | | 闪点(C) | Not applicable | | 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
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| IPTG-RO | Roche | Isopropyl-β-D-thiogalactoside | mol wt 238.3, pkg of 1 g (10724815001), pkg of 5 g (11411446001) | 1004.31元/1 G; 3028.98元/5 G; | 咨询 |
产品说明 一般描述 异丙基-β-D-硫代半乳糖苷(IPTG)是半乳糖的化学类似物,不能被 β-半乳糖苷酶裂解。IPTG 通过与 lacI 阻遏物结合,并改变其构象起作用,其阻止 β-半乳糖苷酶编码基因 lacZ 的阻遏作用。 应用 异丙基-β-D-硫代半乳糖苷(IPTG)通常用于需要诱导 β-半乳糖苷酶活性的克隆程序。它与 X-Gal 或 Bluo-Gal 一起用于蓝白选择的重组细菌菌落,其诱导 lac 操纵子在大肠杆菌中的表达。它已被用于在大肠杆菌中表达重组基因。 IPTG 常用于需要诱导 β-半乳糖苷酶活性的克隆实验。它常与 X-Gal 或 Bluo-Gal 结合使用,用于重组细菌菌落的蓝白筛选,这些菌落可以诱导 lac 操纵子在大肠杆菌中的表达。IPTG 与 lacI 阻遏蛋白结合并改变其构象而发挥作用,防止 β-半乳糖苷酶编码基因 lacZ 的抑制。 其他说明 非代谢性半乳糖类似物。 仅用于生命科学研究。不可用于诊断。 标记 DNA 的数量取决于:
- 模板 DNA 的数量
- 模板 DNA 的纯度
- 模板 DNA 的构象
- 标记片段的平均大小
产品规格 试验时间:50 分钟 比活性:所描述的标准试验将产生 1.8×109 dpm/μg 的比活性,对应于在 30 分钟内与不同底物 DNA 的 65% 掺入。当改变模板 DNA 与标记的 dNTP 的比例时,将得到相似的掺入率,然而得到标记探针的不同水平的比活性。 制备说明 工作浓度:最终浓度应为 0.2 mM。 存储条件(工作溶液):产品水溶液的储备溶液(例如 1M IPTG),储存于 -15 至 -25℃,可稳定保存最长六个月。 样品材料 10 ng 至 3μg 模板 DNA,线性化且长度至少 100 至 200bp 基本信息 | 经验(实验)分子式 | C9H18O5S | | 分子量 | 238.30 | | Beilstein | 4631 | | MDL编号 | MFCD00063273 | | PubChem化学物质编号 | 329815691 |
产品性质 | 分子量 | 238.3 | | 包装 | pkg of 1 g (10724815001) pkg of 5 g (11411446001) | | manufacturer/tradename | Roche | | 运输 | wet ice | | 储存温度 | 2-8℃ | | SMILES string | CC(C)S[C@@H]1OC(CO)[C@H](O)C(O)[C@H]1O | | InChI | 1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1 | | InChI key | BPHPUYQFMNQIOC-NXRLNHOXSA-N |
安全信息 | 储存分类代码 | 11 - Combustible Solids | | WGK | WGK 1 | | 闪点(F) | Not applicable | | 闪点(C) | Not applicable |
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| 958052 | Amethyst | Isopropyl β-D-thiogalactopyranoside | 98%, 试剂级 | 124元/5G; 496元/25G; 1783元/100G; | 咨询 |
基本信息 | 分子式 | C9H18O5S | | 分子量 | 238.30 | | 熔点 | 113 | | 比旋光度([α]20D) | -34.5° to -28.5° (C=1, water) | | MDL编码 | MFCD00063273 | | Beilstein | 4631 | | Merck | 14, 5082 | | EINECS 编号 | 206-703-0 | | 存储条件 | Store at 2-8℃ |
安全信息 | 图形或危害标志 |   | | 提示语 | Warning | | 危险说明 | H315 H319 H335 H351 | | 防范说明 | P281 P305+P351+P338 P302+P340 P312 | | WGK | 3 | | TSCA | 是 |
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| M02726 | Fluorochem | IPTG | >98% | 286元/5g; 1078元/25g; 3080元/100g; | 咨询 |
基本信息 | CAS Number | 367-93-1 | | MDL Number | MFCD00063273 | | Melting Point | 111-118℃ | | Density | 1.37 |
安全信息 | 图形或危害标志 |  | | 危险性说明 | H315: Causes skin irritation. H319: Causes serious eye irritation. H335: May cause respiratory irritation.
| | 防范说明 | P233: Keep container tightly closed. P260: Do not breathe . P261: Avoid breathing . P264: Wash hands thoroughly after handling. P271: Use only outdoors or in a well-ventilated area. P280: Wear . P302 + P352: IF ON SKIN: Wash with plenty of soap and water. P304: IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing.. P304 + P340: IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing. P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P312: Call a POISON CENTER or doctor/physician if you feel unwell. P321: Specific treatment (see relevant procedures. on this label). P332 + P313: If skin irritation occurs: Get medical advice/ attention. P337 + P313: If eye irritation persists: Get medical advice/attention. P340: Remove victim to fresh air and keep at rest in a position comfortable for breathing. P362: Take off contaminated clothing and wash before reuse. P403: Store in a well-ventilated place. P403 + P233: Store in a well-ventilated place. Keep container tightly closed. P405: Store locked up. P501: Dispose of contents/container to in accordance with local regulation .
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