基本信息

技术规格

物性(参考值)

产品说明

一般描述

A potent antagonist of the Sonic Hedgehog (Shh) signaling pathway (IC₅₀ = 20 nM in Shh-LIGHT2 assay and in Ptch1^-l-< cells) that acts by binding to Smoothened (Smo; K_D = 1.2 nM), a distant relative of G protein-coupled receptors. In contrast to cyclopamine, SANT-1 inhibits the activities of both wild type and oncogenic Smo with equal potency (IC₅₀ = 30 nM in SmoA1-LIGHT2 assay).
A potent, cell-permeable antagonist of the Shh (Sonic Hedgehog) signaling pathway (IC₅₀ = 20 nM in the Shh-LIGHT2 assay and in Ptch1^-l- cells) by binding directly to Smoothened (Smo; K_d = 1.2 nM), a distant relative of G protein-coupled receptors. Unlike cyclopamine (Cat. No. 239803), SANT-1 equipotently inhibits the activities of both wild-type and oncogenic Smo (IC₅₀ = 30 nM in SmoA1-LIGHT2 assay).

包装

5 mg in Plastic ampoule
Packaged under inert gas

生化/生理作用

Cell permeable: yes
Primary Target
Shh (Sonic Hedgehog) signaling pathway
Target IC₅₀: 20 nM as antagonist of the Shh (Sonic Hedgehog) signaling pathway in the Shh-LIGHT2 assay and in Ptch1-l- cells
Product does not compete with ATP.
Reversible: no

警告

Toxicity: Standard Handling (A)

重悬

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.







From Catalog:

Desc. Field- added "cell-permeable"

其他说明

Chen, J.K., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 14071.
Frank-Kamenetsky, M., et al. 2002. J. Biol.1, 10.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

产品性质

安全信息

产品说明

应用

使用 SANT-1 处理间变性淋巴瘤激酶(ALK)阳性间变性大细胞淋巴瘤细胞 ² 和慢性淋巴细胞白血病细胞 ³ 会导致细胞死亡。

包装

5, 25 mg in glass bottle

生化/生理作用

SANT-1 在胶质母细胞瘤细胞中通过 Ras/NF-κB 途径抑制 Sonic hedgehog 信号传导，并诱导细胞凋亡。
SANT-1 是一种有效的 sonic hedgehog 信号途径(Shh)拮抗剂，通过与平滑(Smo)受体结合直接抑制。SANT-1 抑制野生型和致癌 Smo 的效率相同。

基本信息

产品性质

安全信息

基本信息